大叶紫薇总黄酮的提取工艺研究

目的]为深入研究大叶紫薇总黄酮的生理功能,对其最佳提取工艺进行研究。方法]采用单因素试验和多因子正交试验对大叶紫薇总黄酮的提取工艺进行初步研究,并比较不同季节对大叶紫薇叶总黄酮含量的影响。结果]单因素试验表明提取工艺的最佳提取条件为料夜比1∶40、乙醇浓度为60%、浸提温度70℃、浸提0.5 h。正交试验表明最佳提取条件为:50%乙醇、料液比1∶40、60℃浸提1.5 h、此条件下提取率为6.71%;3～11月黄酮的含量分别为51.2、49.3、50.4、54.0、65.8、66.1、68.9、70.7和77.0 mg/g,其中11月的总黄酮含量最高。结论]该研究得出最佳提取条件为50%乙醇、料液比1∶40、60℃下浸提1.5 h;11月黄酮的含量最高,为77.0 mg/g。

Study on the Extractive Technology of Total Flavonoids in Lagerstroemia speciosa Leaf

Objective ] For investigation of flavonoids from Lagerstroemia speciosa leaf, the optimal extractive conditions were studies. Meth- od ] The single factor and multi factor orthogonal test were designed to optimize the total tlavonoids extraction process. Also the content of the tatol flavonoids in Lagerstroemia speciosa leaf picked from different seasons were analyzed. ~ Result] The single factor experiment showed that the optimal extractive conditions were as following ： ratio of solid to liquid is 1 ： 40, concentration 60% alcohol, extractive temperature 70 ℃, extractive duration 0.5 h. The orthogonal experimental showed that perfect conditions were ： 50% alcohol, ratio of solid to liquid 1 ： 40, extrac- tive temperature 60 ~C, extractive duration 1.5 h. The ratio of＇extraction was 6.71%. The flavonoids content in the leaves from March to No- vember was 51.2, 49.3, 50.4, 54.0, 65.8, 66.1,68.9, 70.7, 77.0 rag/g, respectively. Conclusion] The optimum extractive condition are 50% alcohol, ratio of solid to liquid is 1：40, extractive temperature 60 ℃C , extractive duration 1.5 h. The leaves from November had the highest flavonoids content, about 77.0 mg/g.