土壤中DDT的微生物修复研究 |
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引用本文: | 潘淑颖,马光辉,常勇,胥慧真,马玉洪.土壤中DDT的微生物修复研究[J].安徽农业科学,2013,41(3):1058-1060. |
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作者姓名: | 潘淑颖 马光辉 常勇 胥慧真 马玉洪 |
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作者单位: | 山东省农业环境保护总站,山东济南,250100;山东省安丘市农业局,山东安丘,262100;山东师范大学山东省可持续发展研究中心,山东济南,250014;济南碧霄环保节能科技有限公司,山东济南,250013 |
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基金项目: | 山东省科技攻关项目“土壤中持久性有机污染物原位降解条件的优化研究”(2009GG10006018);山东省教育厅科技计划项目“基于GIS的矿区土壤重金属污染空间分布规律及评价研究”(J11LE116);“基于GIS的山东省土壤农药污染空间分布规律及复合降解研究”(J10LE13) |
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摘 要: | 目的]为了解土壤中有机氯类农药DDT降解过程中土壤微生物所起的作用。方法]采取从土壤中分离培养土著微生物的办法,筛选出耐受DDT的菌株,进行强化培养。在提供共代谢底物的条件下,研究它们与白腐真菌对土壤中DDT的降解效果。结果]在DDT的微生物降解过程中,加入有机质作为共代谢底物,可以使它们的降解效果大幅提高,而从土壤中筛选出的鞘氨醇杆菌属细菌(Sphingobacterium sp.)DB02,即使不加入有机质作为共代谢底物,对DDT仍有较好的降解效果。结论]与从土壤中分离出的土著微生物相比,白腐真菌对土壤中的DDT有更加显著的降解效果。
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关 键 词: | 土壤 DDT 微生物 降解 共代谢底物 |
Study of Microbes Degradation of DDT in Soil |
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Institution: | PAN Shu-ying et al(Shandong Province Environmental Environmental Protection Chief Station,Jinan,Shandong 250100) |
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Abstract: | Objective ] The research aimed to understand the role of microbes played in the process of the degradation of DDT in soil. Meth- od ] Indigenous micro-organisms were isolated and cultivated so that the strains that could tolerate DDT were screened and amplified. Then, the strains of Phanerochaete sp. , provided with co-metabolism substrate, were placed into the soil to degrade DDT, and the strains and the effect of the degradation were studied. Result] In the process of the degradation of DDT by the microbes, the degradation could be more effective if organic matter as the co-metabolism substrate was added. It was also obvious in the experiment that Sphingobacterium sp. , screened and classified before, degraded DDT in an excellent efficiency even if organic co-metabolism substrate wash' t supplied. Conclusion ] Compared with indigenous microbes isolated from soil, Phanerochaete sp. has better performance in the degradation of DDT in soil. |
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Keywords: | Soil DDT Microbes Degradation Co-metabolism substrate |
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