| 健康仔猪肠道内乳酸菌的分离与鉴定 |
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| 引用本文： | 顾甜甜,秦晓冰,单虎,温建新,周保琨.健康仔猪肠道内乳酸菌的分离与鉴定[J].安徽农业科学,2013(2):630-632. |
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| 作者姓名： | 顾甜甜 秦晓冰 单虎 温建新 周保琨 |
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| 作者单位： | 青岛农业大学动物科技学院,山东青岛,266109;山东省即墨市畜牧兽医局,山东即墨,266200 |
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| 基金项目： | 山东省优秀中青年科学家科研奖励基金(BS2011SW010) |
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| 摘 要： | 目的]从健康仔猪肠道内分离并鉴定乳酸菌。方法]结合细菌形态学、生理生化试验以及16S rRNA序列分析等鉴定方法。结果]共分离到10株疑似乳酸菌,有9株菌株属于乳杆菌目,其中C1、C2、C6属于链球菌属;C3、F2、F3为约氏乳杆菌;C4、F4为屎肠球菌;C5为嗜酸乳杆菌;结论]该研究可为仔猪微生态制剂的制备提供菌株基础。
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| 关 键 词： | 乳酸菌 分离 鉴定 生化试验 16S rRNA序列分析 |
Separation and Identification of Lactobacillus from the Intestinal Tract of Healthy Piglet |
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| Institution: | GU Tian-tian et al(College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong 266109) |
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| Abstract: | Objective] Lactobacillus were separated and identified from intestinal tract of healthy piglet.Method] The Lactobacillus were identified through bacteria morphology,physiological and biochemical tests and 16S rRNA sequences analysis.Result] 9 strains of 10 suspected Lactobacillus are Lactobacillales,and C1,C2,C3 belong to Streptococcus,C2,F2,F3 are Lactobacillus johnsonii,C4,F4 are Enterococcus faecium and C5 is Lactobacillus acidophilus.Conclusion]The study provides a bacteria strain foundation for preparation of piglet probiotics. |
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| Keywords: | Lactobacillus Separation Identification Biochemical testing 16S rRNA sequences analysis |
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