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Flagellin glycosylation is ubiquitous in a broad range of phytopathogenic bacteria |
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| Authors: | Yuki Ichinose Fumiko Taguchi Masanobu Yamamoto Mayumi Ohnishi-Kameyama Tatsuo Atsumi Masako Iwaki Hiromi Manabe Mio Kumagai Quan Thanh Nguyen Chi Linh Nguyen Yoshishige Inagaki Hiroshi Ono Kazuhiro Chiku Tadashi Ishii Mitsuru Yoshida |
| Affiliation: | 1. The Graduate School of Environmental and Life Science, Okayama University, 1-1-1 Tsushima-naka, Okayama, 700-8530, Japan 2. National Food Research Institute, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan 3. Department of Radiological Technology, School of Health Sciences, Gifu University of Medical Science, 795-1 Ichihiraga Nagamine, Seki, Gifu, 501-3892, Japan 4. Division of Biological Science, Graduate School of Science, Nagoya University, Nagoya, Aichi, 464-8602, Japan 6. The Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-naka, Okayama, 700-8530, Japan 5. Faculty of Agriculture, Okayama University, 1-1-1 Tsushima-naka, Okayama, 700-8530, Japan |
| Abstract: | Glycosylation of flagellin is known to be involved in filament stabilization, motility, and virulence in Pseudomonas syringae. Here we investigated flagellin glycosylation in other phytopathogenic bacteria. Analyses of deduced amino acid sequences, glycostaining, and molecular masses of purified flagellins revealed that flagellins from all phytopathogenic bacteria investigated were glycosylated. Furthermore, the flagellin in a glycosylation-defective mutant of Xanthomonas campestris pv. campestris (Xcc) had a reduced molecular mass, and motility and virulence of the mutant toward host leaves decreased. These results suggest that flagellin glycosylation is ubiquitous in most phytopathogenic bacteria and that flagellin glycosylation is required for virulence in Xcc. |
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