Mapping neutralizing and non-neutralizing epitopes on the capsid protein of feline calicivirus |
| |
Authors: | Geissler K Schneider K Truyen U |
| |
Institution: | Institute for Medical Microbiology, Infectious and Epidemic diseases, Ludwig Maximilians University Munich, Germany. |
| |
Abstract: | Neutralizing epitopes on feline calicivirus (FCV) capsid protein were mapped using chimeric capsid proteins recombinant between two FCV isolates that do not show any cross-neutralization. The three chimeric proteins examined were expressed in murine L929 cells employing an MVA/T7 vaccinia virus expression system and inoculated into major histocompatibility complex haplotype-matched C3/HN mice. Based on the neutralizing antibody titre the neutralizing epitope(s) could be mapped to the 5' hypervariable region of the E region or potentially to the C region. The epitopes of some non-neutralizing antibodies were mapped with the same chimeric proteins to the regions B or D and F of the FCV capsid protein. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |