野艾蒿中双四氢呋喃类木脂素的提取分离及其抑菌活性

为筛选新型植物源杀菌剂，对野艾蒿Artemisia lavandulaefolia DC. 地上组织中的化学成分进行了研究。采用甲醇提取、液-液萃取、柱层析及半制备高效液相色谱等技术从乙酸乙酯浸膏中分离得到6个化合物；利用质谱及核磁共振等波谱技术确定这6个化合物分别是epiashchantin ( 1 )、ashantin ( 2 )、sesartemin ( 3 )、epimagnolin A ( 4 )、epiyangambin ( 5 ) 和diayangambin ( 6 )，均属于双四氢呋喃类木脂素。采用菌丝生长速率法测定了其抑菌活性，结果表明:化合物 1 和 3 对番茄早疫病菌Alternaria solani的菌丝生长表现出明显抑制作用，EC₅₀值分别为14.3和11.0 μg/mL；化合物 3 、 5 和 6 对玉米纹枯病菌Rhizoctonia solani的菌丝生长也表现出抑制作用，EC₅₀值分别为17.4、42.6和54.3 μg/mL。

Bistetrahydrofuran lignans isolated from Artemisia lavandulaefolia DC and their antifungal activities

In order to discover novel botanical fungicides, the chemical constituents of the aerial parts of Artemisia lavandulaefolia DC. were investigated. The aerial parts were extracted with methanol. By using liquid-liquid extraction, column chromatography, high performance preparative liquid chromatography and other techniques, 6 pure compounds were finally isolated from the ethyl acetate phase. The compounds were identified as bistetrahydrofuran lignans, including epiashchantin ( 1 ), ashantin ( 2 ), sesartemin ( 3 ), epimagnolin A ( 4 ), epiyangambin ( 5 ) and diayangambin ( 6 ), according to NMR and MS data. The mycelium growth rate method was used to determine the antifungal activities. The results showed that compound 1 and 3 significantly inhibited the growth of Alternaria solani with the EC₅₀ value of 14.3 μg/mL and 11.0 μg/mL, respectively. Compound 3 , 5 and 6 also showed inhibitory effect against the mycelia growth of Rhizoctonia solani with the EC₅₀ value of 17.4, 42.6 and 54.3 μg/mL, respectively.