泽泻汤加味方对盐敏感性HBZY21细胞中AngII-NADPH-ROS信号通路的影响

目的 探讨泽泻汤加味方对盐敏感性肾小球系膜细胞中AngII-NADPH-ROS信号通路的作用机制。方法 采用高盐和AngII诱导大鼠肾小球系膜细胞的方法建立盐敏感性高血压体外细胞模型，设正常组、模型组、阳性药缬沙坦组、泽泻汤加味方（高、中、低剂量）组。CKK-8法测定细胞活性；RT-qPCR方法检测细胞中Agtr1a、Cyba、NOX4的mRNA表达水平；Western blot方法检测细胞中AT1R、P22、P47、NOX4的蛋白质表达水平；活性氧检测试剂盒检测细胞中ROS水平。结果 与正常组比较，模型组细胞中Agtr1a、Cyba、NOX4的mRNA表达水平、AT1R、P22、P47、NOX4蛋白表达水平以及ROS表达水平显著升高（P<0.05）。与模型组比较，缬沙坦组及泽泻汤加味方中剂量组Agtr1a、Cyba、NOX4的mRNA表达水平、AT1R、P22、P47、NOX4蛋白表达水平以及ROS表达水平显著降低（P<0.05）。不同浓度泽泻汤加味方组间，中剂量组对AngII-NADPH-ROS信号通路的下调作用最为明显。结论 泽泻汤加味方对盐敏感性高血压肾损害的保护作用可能与抑制AngII-NADPH-ROS信号通路有关。

Impact of Modified Zexie Decoction on the AngII-NADPH-ROS Signaling Pathway in Salt-Sensitive HBZY21 cells

Objective To investigate the effect of modified Zexie Decoction on the angiotensin II (AngII)-NADPH-reactive oxygen species (ROS) signaling pathway in salt-sensitive glomerular mesangial cells. Methods An in vitro cell model of salt-sensitive hypertension was established by inducing rat glomerular mesangial cells with high salt and AngII. Cells were divided into normal group, model group, positive valsartan group, and high-, medium-, and low-dose modified Zexie Decoction groups. CKK-8 assay was used to determine the cell viability. RT-PCR was used to determine the mRNA expression levels of Agtr1a, Cyba, and NOX4. Western blot was used to determine the protein expression levels of AT1R, P22, P47, and NOX4 in cells. The ROS levels in cells were measured by a ROS assay kit. Results Compared with the normal group, the model group had significantly higher mRNA levels of Agtr1a, Cyba, NOX4, protein levels of AT1R, P22, P47, and NOX4, and ROS levels (P<0.05). Compared with the model group, the valsartan group and the medium-dose modified Zexie Decoction group had significantly lower mRNA levels of Agtr1a, Cyba, and NOX4, protein levels of AT1R, P22, P47, and NOX4, and ROS levels (P<0.05). Among the three modified Zexie Decoction groups, the medium-dose group had the greatest downregulation of the AngII-NADPH-ROS signaling pathway. Conclusion The protective effect of modified Zexie Decoction against renal injury induced by salt-sensitive hypertension might relate to the inhibition of the AngII-NADPH-ROS signaling pathway.