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| 海洋细菌的分离、鉴定及抗烟草花叶病毒活性分析 | |
| 引用本文： | 韩凤英,谢荔岩,连玲丽,林奇英.海洋细菌的分离、鉴定及抗烟草花叶病毒活性分析[J].福建农林大学学报(自然科学版),2012,41(3):281-285. |
| 作者姓名： | 韩凤英 谢荔岩 连玲丽 林奇英 |
| 作者单位： | 1. 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州350002;山东省农业管理干部学院,山东济南250100 2. 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州350002 3. 福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福建福州350002;福建农林大学生命科学学院,福建福州350002 |
| 基金项目： | 福建省青年人才项目，福建省发改委资助项目 |
| 摘 要： | 从福建霞浦海域分离、筛选到6株对烟草花叶病毒(TMV)具有体外钝化活性的菌株,其中,菌株9和10的抑制率较高,分别达95.26%和92.18%.进一步测定2株菌对TMV的抑制增殖活性,结果表明,菌株9和10可明显抑制TMV的增殖,其抑制率分别为36.29%和35.08%.通过对2株菌的形态特征、生理生化反应及16S rDNA序列的分析,将菌株9鉴定为蜡样芽孢杆菌,菌株10鉴定为水生海洋芽孢杆菌. |
| 关 键 词： | 海洋细菌 抗烟草花叶病毒活性 分离鉴定 |
Isolation, identification and antiviral activity of marine bacteria against Tobacco mosaic virus |
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| HAN Feng-ying , XIE Li-yan , LIAN Ling-li , LIN Qi-ying.Isolation, identification and antiviral activity of marine bacteria against Tobacco mosaic virus[J].Journal of Fujian Agricultural and Forestry University,2012,41(3):281-285. | |
| Authors: | HAN Feng-ying XIE Li-yan LIAN Ling-li LIN Qi-ying |
| Institution: | 1(1.Fujian Key Laboratory for Plant Virology,Institute of Plant Virology,Fujian Agriculture and Forestry University,Fuzhou, Fujian 350002,China;2.Shandong Agricultural Administrators College,Jinan,Shandong 250100,China; 3.College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
| Abstract: | Six marine bacteria isolated from tidal marine mud in Xiapu,Fujian,exhibited activities against Tobacco mosaic virus(TMV).Among them,the strain 9 and 10 showed strong antiviral activities in vitro,resulting in a inhibition rate of 95.26% and 92.18% respectively.Further tests on their antiviral activities in vivo indicated that both strains inhibited the reproduction of TMV particle and the inhibition rates were 36.29% and 35.08% respectively.According to the morphological physiological characters and 16S rDNA sequence,strain 9 was identified as Bacillus cereus,strain 10 was identified as Bacillus aquimaris. |
| Keywords: | marine bacteria anti-TMV activity isolation and identification |
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