| 甘薯二羟基黄酮醇还原酶基因的克隆及在大肠杆菌中的表达 |
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| 引用本文： | 赵宝杰,侯夫云,王庆美,李爱贤,张立明. 甘薯二羟基黄酮醇还原酶基因的克隆及在大肠杆菌中的表达[J]. 分子植物育种, 2008, 6(3): 583-586 |
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| 作者姓名： | 赵宝杰 侯夫云 王庆美 李爱贤 张立明 |
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| 作者单位： | 青岛农业大学植物科技学院,青岛,266109;山东省农业科学院作物所,济南,250100 |
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| 基金项目： | 国家高技术研究发展计划(863计划) , 国家科技支撑计划 , 山东欧省良种工程项目 , 山东农业科学院高技术自主创新和青年基金 , 山东农业科学院青年基金 |
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| 摘 要： | 根据其它作物的二羟基黄酮醇还原酶（DFR）基因的保守序列设计引物,利用RT-PCR方法从甘薯块根中分离出花青素合成相关基因DFR基因,并定名为ibDFR。该基因全长1232bp,编码398aa。核甘酸序列分析表明,与牵牛相似系数达85%,与烟草的相似系数达到67%,与马铃薯的相似系数达75%。将ibDFR连接到pET30a（＋）载体中,检测ibDFR在大肠杆菌中的表达。实验结果表明ibDFR蛋白质分子量大小与推算一致,能在大肠杆菌中转录表达。
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| 关 键 词： | 甘薯 花青素 DFR基因 克隆 |
Cloning of Dihydroflavonol 4-reductase Gene from Sweetpotato and its Expression in E.coli |
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| Zhao Baojie,Hou Fuyun,Wang Qingmei,Li Aixian,Zhang Liming. Cloning of Dihydroflavonol 4-reductase Gene from Sweetpotato and its Expression in E.coli[J]. Molecular Plant Breeding, 2008, 6(3): 583-586 |
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| Authors: | Zhao Baojie Hou Fuyun Wang Qingmei Li Aixian Zhang Liming |
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| Affiliation: | Zhao Baojie 1 Hou Fuyun 2 Wang Qingmei 2 Li Aixian 2 Zhang Liming 21 Plant Science , Technology College,Qindao Agricultural University,Qindao,266109,2 Crop Research Institute,Sh,ong Academy of Agricultural Sciences,Jinan,250100 |
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| Abstract: | According to the conserved sequences of dihydroflavonol 4-reductase (DFR) genes from other plants, primers were designed to amplify DFR gene from sweet potato, and the gene was deginated ibDFR in this paper. The gene have 1 232 bp necleounide and encoded 398 aa. Sequence comparison revealed that ibDFR sequence shared homologies of 85%, 67% and 75% with those of Ipomoea purpurea, tobacco and potato. The opening read fragment of ibDFR were inserted with pET30a (+) using lingase to detect the expression of ibD... |
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| Keywords: | Sweet potato Anthocyanin DFR gene Clone |
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