高效液相色谱法测定大黄藤中生物碱的研究

目的]研究用高效液相色谱测定大黄藤中小檗碱、巴马汀、药根碱含量。方法]样品中的生物碱采用乙腈（含0．1％的磷酸和0．1％的十二烷基硫酸钠）溶液提取，以ZORBAX Stable Bound C18（3．9mm×250mm，5．0μm）色谱柱为固定相，乙腈-0．1％磷酸缓冲溶液（60：40，含0．1％的十二烷基硫酸钠）为流动相分离；紫外检测器265nm波长检测测定，流动相流速1．0ml／min，柱温为25℃，进样量20ml。结果]药根碱、巴马汀碱、小檗碱平均回收率分别为102．8％、98．4％、97．6％，RSD分别为1．60％、1．80％、1．50％。结论]方法灵敏度高，重现性好，可用于大黄藤中生物碱的测定。

Study on the Determination of Alkaloid in Fibraurea recisa Pierre with High Performance Liquid Chromatography

Objective]The determination method of the jatrorrhizine, palmatine and berberine in Fibraurea recisa Pierre was researched with high performance liquid chromatography(HPLC). Method]The alkaloids were extracted from the sample with the acetonitrile solution containing 0.1% phosphoric acid and SDS. The alkaloids was separated on a ZORBAX Stable Bound (3.9 mm×250 mm, 5 μm) chromatographic column with acetonitrile-0.1% phosphoric acid(60∶ 40 and containing 0.1% SDS) as mobile phase at a the flow-rate of 1.0 ml/min, the temperature of 25 ℃ and used amount of 20 ml, and then, monitored with the photodiode array detector at 265 nm. Results]The recoveries rate of jatrorrhizine, palmatine and berberine was 102.8%, 98.4% and 97.6%, and the relative standard deviation(RSD) was 1.60%, 1.80% and 1.50%, respectively. Conclusion]This method was with high sensitivity and good reproducibility, which could be applied in the determination of alkaloids in Fibraurea recisa Pierre.