辛伐他丁对2型糖尿病大鼠血管内皮VCAM-1和MCP-1mRNA和蛋白表达的影响

目的了解辛伐他丁对2型糖尿病大鼠血管内皮细胞黏附分子-1(VCAM-1)和单核细胞趋化因子-1(MCP-1)的影响。方法30只SD大鼠随机分为正常组(NC)、糖尿病组(DMC)、辛伐他丁治疗组(DMT),每组各10只大鼠。喂养12周后抽血测定血脂全套,取主动脉标本以免疫组化和半定量RT-PCR法分别检测VCAM-1和MCP-1的mRNA和蛋白表达。结果DMT组大鼠血管内皮VCAM-1和MCP-1的mRNA和蛋白表达明显降低,与DMC组比较差异有统计学意义(P<0.05)。结论辛伐他丁可减少糖尿病大鼠血管内皮VCAM-1和MCP-1的表达,减轻血管内皮局部的炎症反应。

Effect of simvastatin on endothelial expression of VCAM-1 and MCP-1 in rat model of type 2 diabetes mellitus

Objective To investigate the effect of simvastatin on endothelial expression of vascular cell adhesion molecule-1 (VCAM-1) and monocyte chemoattractant protein-1 (MCP-1) in rats with type 2 diabetes mellitus. Methods Thirty SD rats were evenly randomized to normal group, diabetic group and simvastatin group. Serum levels of total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C) and triglyceride (TG) were assayed, and the aortic expression of VCAM-1 and MCP-1 was detected by immunohistochemistry and RT-PCR in 3 groups after 12 weeks. Results The expression of VCAM-1 and MCP-1 protein and mRNA in aortic endothelium was significantly lower in simvastatin group than that in diabetic group (P<0.05). Conclusion Simvastatin could attenuate the endothelial inflammation by downregulation of VCAM-1 and MCP-1 expression in diabetic rat model.