| 海南野生兰内生细菌调查与初步分析 |
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| 引用本文： | 王丰,莫饶. 海南野生兰内生细菌调查与初步分析[J]. 安徽农业科学, 2009, 37(16): 7330-7331 |
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| 作者姓名： | 王丰 莫饶 |
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| 作者单位： | 海南大学,海南儋州,571737;海南大学,海南儋州,571737 |
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| 基金项目： | 海南大学作物遗传重点学科资助项目 |
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| 摘 要： | [目的]对海南部分野生兰品种进行内生细菌的分离和鉴定,希望能分离出有拮抗作用的内生细菌。[方法]利用组织培养法来进行内生细菌的分离,用16 S rDNA方法进行该内生细菌的分子生物学鉴定。[结果]分离出有7株内生菌分离物具有一定的拮抗活性,其中有2株拮抗作用较明显,分别命名为WF1和WF2。利用不同浓度的利福平对这两株内生菌分离物进行抗生素选择压力筛选,获得耐抗生素菌株WF1,进而利用该菌株进行回接试验。[结论]提取WF1的总DNA后,进行PCR、产物的回收、连接及转化。阳性克隆从CW34668引物端测序,测得的1418 bp的片段可在GenBank库中的各枯草芽胞杆菌16 S rDNA基因找到同源序列,且相似性达到99.5%,确定该菌属于芽胞杆菌属。
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| 关 键 词： | 野生兰 内生细菌 分离 |
Investigation and Analysis of the Endophyte Bacteria of Wild Orchids in Hainan Province |
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| WANG Feng et al. Investigation and Analysis of the Endophyte Bacteria of Wild Orchids in Hainan Province[J]. Journal of Anhui Agricultural Sciences, 2009, 37(16): 7330-7331 |
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| Authors: | WANG Feng et al |
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| Affiliation: | WANG Feng et al(Hainan University,Danzhou,Hainan 571737) |
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| Abstract: | [Objective] The antagonistic activity of endophyte bacteria was hoped to be isolated through their isolation and identification from some wild orchids varieties in Henan.[Method] The endophyte bacteria were isolated with the tissue culture method and the biological character of them was identified with the method of 16 S rDNA.[Result] The isolators from 7 bacterial strains had the antagonistic activity,among which,the prominent antagonistic activity appeared in 2 strains,named as WF1 and WF2.The WF1 and WF2... |
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| Keywords: | Wild orchids Endophyte bacteria Isolation |
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