Levamisole and its influence on the immune response of lambs |
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Authors: | W Cabaj M Stankiewicz W E Jonas L G Moore |
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Institution: | (1) AgResearch, Wallaceville Animal Research Centre, PO Box 40-063, Upper Hutt, New Zealand |
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Abstract: | Ten parasite-free lambs were drenched with 8 mg/kg of levamisole on days 0 and 28 and were injected with human erythrocytes and ovalbumin one day after each drench. Ten other antigen-injected lambs were not drenched with anthelmintic as controls. Lymphocytes from the control and drenched lambs were culturedin vitro with RPMI 1640 plus 5% fetal calf serum (FCS), with 50% autologous serum only, with concanavalin A (Con A) or with phytohaemagglutinin (PHA). Decreased blastogenesis was observed in cells from the drenched lambs cultured in the presence or absence of mitogen and was most obvious when 50% autologous serum was used, particularly with PHA, and when lymphocytes were collected 3 and 7 days after the first and 3 days after the second antigen injection. There were no significant changes in antibody titres between the groups. Decreased serum complement activity was seen 3 days after the second antigen injection in the drenched lambs. Although there was a significant reduction in the serum insulin-like growth factor I levels 4 days after each levamisole drench, the drenched lambs gained significantly more weight than the non-drenched control lambs.Abbreviations Con A
concanavalin
- EIA
enzyme immunoassay
- FCS
fetal calf serum
- GH
growth hormone
- IGF-I
insulin growth factor I
- PHA
phytohaemagglutinin |
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Keywords: | cellular immunity humoral immunity immunomodulation levamisole sheep |
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