| 紫外线诱变选育高产纳豆激酶菌株的研究 |
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| 引用本文： | 夏丽,沙维,张丽萍.紫外线诱变选育高产纳豆激酶菌株的研究[J].农产品加工．学刊,2010(4). |
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| 作者姓名： | 夏丽 沙维 张丽萍 |
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| 作者单位： | 黑龙江八一农垦大学食品学院; |
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| 基金项目： | 黑龙江省农垦总局科技计划项目(HNKXIV-06-05a); 黑龙江省研究生创新科研项目(YJSCX2009-125HLJ) |
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| 摘 要： | 为了获得一株产纳豆激酶(NK)活力较强的菌株。以纳豆激酶活力为评价指标,采用紫外线诱变育种的方法和单因素实验方法,确定紫外线诱变剂量,对原始菌株进行诱变育种,并采用纤维蛋白平板法,测定菌株发酵的纳豆激酶活力。试验结果表明,20W紫外灯距离30cm照射240s的诱变,效果较佳。经过诱变及菌种筛选所获得的高产菌株U-5发酵生产的纳豆激酶活力比原始菌株T-3提高了8.81%。经15代传代试验,U-5高效突变株生产的纳豆激酶活力均稳定在5440.37IU/mL以上。
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| 关 键 词： | 纳豆激酶 紫外线 诱变 |
Breeding of NK producing Bacillus Subtilis by Ultraviolet Radiation |
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| Xia Li,Sha Wei,Zhang Liping.Breeding of NK producing Bacillus Subtilis by Ultraviolet Radiation[J].Nongchanpin Jlagong.Xuekan,2010(4). |
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| Authors: | Xia Li Sha Wei Zhang Liping |
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| Institution: | Xia Li,Sha Wei,*Zhang Liping(College of Food Science,Heilongjiang August-first Land Reclamation University,Daqing,Heilongjiang 163319,China) |
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| Abstract: | Objective] We obtained a new mutant with higher growth rate and high capability of producing NK after ultraviolet radiation mutation.Methods] Using NK production as evaluation index,we determined conditions by single factor experiment.Results] A high-yield strain U-5 was obtained through mutating by ultraviolet radiation irradiation 240 seconds(20 W,30 cm).Conclusion] After ultraviolet radiation mutation and repeatedly screening,a mutat of Bacillus subtilis U-5 whose NK concentration was 8.81% times of ... |
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| Keywords: | NK ultraviolet radiation mutation |
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