| 孜然芹下胚轴胚性愈伤组织的诱导及再生植株的研究 |
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| 引用本文： | 高杰,陈小华,高翔,孙军.孜然芹下胚轴胚性愈伤组织的诱导及再生植株的研究[J].新疆农业科学,2007,44(6):866-869. |
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| 作者姓名： | 高杰 陈小华 高翔 孙军 |
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| 作者单位： | 新疆农业大学园艺学院,乌鲁木齐,830052;新疆维吾尔自治区种子总站,乌鲁木齐,830038 |
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| 摘 要： | 以孜然芹下胚轴为外值体,研究了在不同种类和浓度激素处理下产生愈伤组织,经继代增殖成胚性愈伤组织,在分化培养基上,胚性愈伤组织进一步分化,得到了再生植株.结果表明:下胚轴在光照时间16 h/d,MS+2,4-D 0.5 mg/L的愈伤组织诱导启动培养基中,20 d愈伤诱导率达100;,继代培养基为MS+NAA 0.5 mg/L+6-BA 2 mg/L培养基中,增殖率达80;,在MS+6-BA 3.0 mg/L+NAA 0.5 mg/L培养基中光照16 h/d培养30 d,可见再生植株,再生率为18.75;.
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| 关 键 词： | 孜然芹 下胚轴 愈伤组织诱导 再生植株 |
| 文章编号： | 1001-4330（2007）06-0866-04 |
| 收稿时间： | 2007-02-27 |
| 修稿时间： | 2007-07-19 |
Research on Embryonic Callus Induction and Regenerative Plant of Hypocotyl of Cuminum cyminum L. |
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| GAO Jie,CHEN Xiao-hua,GAO Xiang,SUN Jun.Research on Embryonic Callus Induction and Regenerative Plant of Hypocotyl of Cuminum cyminum L.[J].Xinjiang Agricultural Sciences,2007,44(6):866-869. |
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| Authors: | GAO Jie CHEN Xiao-hua GAO Xiang SUN Jun |
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| Abstract: | The callus were induced from hypocotyls of Cuminum cyminum L..The rate of the callus induction of hypocotyl culture was 100% for a period of 20d with 16 hour photoperiod each day on MS basal medium supplemented with 2,4-D 0.5 mg/L.In the second culture,the rate of growth of callus could reach 80% with NAA 0.5 mg/L and BA 2.0 mg/L.The plant generation was founded on MS basal medium supplemented with 6-BA 3.0 mg/L NAA0.5 mg/L,the rate was 18.75%. |
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| Keywords: | Cuminurn cyminum L hypocotyl callus induction regeneration plant |
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