一种小鸡体液免疫应答能力指示系统的建立

本研究用兔、山羊、绵羊、黄牛、猪、鹅、鸭的红细胞(RBC)作为抗原,分别免疫小鸡,并用直接微量血球凝集(HA)试验测定鸡所产生的体液免疫应答。选用能刺激产生较高抗体水平的鹅红细胞(GRBC)作为指示系统,在7、14天龄分别接种试验鸡,检测(1)传染性囊病(IBD)病毒及疫苗,(2)马立克氏病(MD)病毒;(3)火鸡疱疹病毒(HVT)疫苗等接种1天龄小鸡后,对鸡体液免疫应答能力的影响。 结果表明,IBD—PBG_(98)毒株无免疫抑制作用;IBD—野毒株引起严重的免疫抑制效应;HVT疫苗和MD病毒对7天龄接种了GRBC的小鸡抗体产生有明显影响。同时对鸡的法氏囊进行组织学检查,取得相应的结果。然而,IBD中和抗体滴度并没有显出这种与法氏囊组织破坏呈正相关的关系。 试验证明本指示系统简便有效,易于推广应用。

ESTABLISHMENT OF AN INDICATOR SYSTEM FOR ASSESSMENT OF HUMORAL IMMUNE RESPONSE IN CHICKS

In the present study, red blood cells from each of the following species,rabbit,goat, sheep, cattle, swine, goose and duck were used as antigen to immunizechicks, and the humoral immune response determined by a direct micro-haemag-glutination test.Goose red blood cells(GRBC) elicited antibodies which gave aclear-cut and strong antibody reaction, and thus was chosen as the indicatorsystem.Chicks were inoculated with GRBC at 7 and 14 days of age respectively toassess the immunological responsiveness of the birds exposed at the age of oneday to (1) infectious bursal disease (IBD) virus and vaccine, (2) Marek'sdisease (MD) virus, and (8) Herpesvirus of turkey (HVT) vaccine.The results showed that the IBD-PBG98 strain produced no immunosuppression,the IBD-virulent strain led to serious immunodepressive effect. The MD virusand HVT vaccine significantly affected the production of haemagglutinatingantibodies in those birds which were injected with GRBC at 7 days of age.parallel histological examination of the bursa Pabricii revealed changes whichwere in agreement with the above observations. However, the neutralizingantibody titres to IBD virus did not show this correlation.This study demonstrated that the GRBC indicator system for evaluating humoralimmune response in chicks was simple, effective and easy to popularize.