| 不同浓度6-BA对“红标无核”葡萄茎段培养的影响 |
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| 引用本文： | 李永辉,白远国,邓红云,陈豪.不同浓度6-BA对“红标无核”葡萄茎段培养的影响[J].安徽农业科学,2007,35(15):4452-4453. |
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| 作者姓名： | 李永辉 白远国 邓红云 陈豪 |
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| 作者单位： | 1. 荆州职业技术学院,湖北荆州,434100
2. 湖北省荆楚种业,湖北荆州,434100 |
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| 摘 要： | 以1/2MS、MS和B5为基本培养基研究了不同浓度的6-BA对“红标无核”葡萄茎段培养的影响。结果表明,采用70%的乙醇20 s和1.0 g/kg升汞5 min对外植体消毒效果最理想。芽诱导的6-BA最佳浓度为2.5 mg/L,在MS培养基上芽的诱导率达到90%,在B5培养基上诱导率为80%;在MS培养基中,6-BA浓度为1.0 mg/L,IAA浓度为0.5 mg/L时,芽的分化与增殖均为最佳;在1/2MS生根培养基中,IBA浓度为1.0 mg/L时生根率达84%,平均根数为5条,根生长良好。
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| 关 键 词： | 6-BA 红标无核 葡萄 组织培养 |
| 文章编号： | 0617-6611（2007）15-04452-02 |
| 修稿时间： | 2007-02-28 |
Effect of the Different Concentration of 6-BA on Stem Segment Cultlning of Hongbiao Seedless Grape |
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| LI Yong-hui,et al.Effect of the Different Concentration of 6-BA on Stem Segment Cultlning of Hongbiao Seedless Grape[J].Journal of Anhui Agricultural Sciences,2007,35(15):4452-4453. |
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| Authors: | LI Yong-hui |
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| Institution: | Jingzhou College of Vocation Technique,Jingzhou,Hubei 43400 |
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| Abstract: | The apical stem and stem segment with axilla bud of Hongbiao Seedless Grape were cultured on the basic medium with different concentration of 6-BA.Results showed that using 70% ethanol for 20 s and 1.0 g/kg Hg for 5 min got optimum effect on sterilizing explants.The optimum concentration of 6-BA was 2.5 mg/L in inducing bud,with the bud induction of 90% on MS medium and 80% on B5 medium.When the 6-BA concentration was 1.0 mg/L,IAA concentration was 0.5 mg/L,the differentiation and multiplication of the induced buds were optimum.On the 1/2 MS rooting medium,when IAA concentration was 0.5 mg/L,the root grown well and the rooting reached 84%,with roots number of 5 on average. |
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| Keywords: | 6-BA Hongbiao Seedless Grape Tissue culture |
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