Clonal propagation of Acacia saligna by shoot tip culture |
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| Authors: | M. N. Barakat M. H. El-Lakany |
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| Affiliation: | (1) Tissue Culture Laboratory, Department of Crop Science, Faculty of Agriculture, University of Alexandria, Egypt;(2) Tissue Culture Laboratory, Desert Development Center, The American University in Cairo, Egypt |
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| Abstract: | Summary Multiple shoots buds were obtained successfully from shoot tips of Acacia saligna by placing explants into solidified Murashighe & Skoog (1962) medium (MS medium) supplemented with 5.0 to 9.0 mg/L BAP. Sequential culture treatment was highly effective for shoot elongation using MS medium containing 0.3 mg/L BAP and 0.2 mg/L IAA. The shoots rooted best on MS medium supplemented with 2.0 mg/L IBA. Plantlet survival after transfer to soil was more than 90%. The shoot proliferation method described could be used for the mass clonal propagation of selected genotypes. |
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| Keywords: | Acacia saligna clonal propagation shoot tip culture forage crop |
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