中国东北大豆与日本大豆品种PEPCase基因的多态性

Soybeans in Northeast of China are one of the most importantgenetic resources in the world.In order to use themeffectively insoybean breeding,it is necessary to evaluate the maincharacteristics,especially the quality characteristics such as seedprotein content.It is reported that the PEPCase activity in soybeanseeds is positively correlated with the seed protein content andnegatively correlated with the lipid content1].PEPCase is alsopresent in seeds of different species2~4],and may play…

Polymorphism of Phosphoenolpyruvate Carboxylase Gene in Soybean Cultivars from Northeastern China and Japan

Soybeans in Northeast of China are one of the most important genetic resources in the world.In order to use them effectively in soybean breeding, it is necessary to evaluate the main characteristics,especially the quality characteristics such as seed protein content.It is reported that the PEPCase activity in soybean seeds is positively correlated with the seed protein content and negatively correlated with the lipid content1].PEPCase is also present in seeds of different species2～4],and may play an important role in auino acid biosynthesis.PEPCase was detected in the protein bodies of developing wheat grains,possibly contributing to storage-protein biosynthesis5]. A strong correlation between PEPCase activity and protein concentration in seeds of 13 soybean cultivars1]indicates a possible rate-limiting role of the enzyme in seed storage-protein accumulation.In soybean,PEPCase is encoded by a small family of at least four highly homologous genes6]and PEPCase catalyzes the carboxylation of phosphonenopyruvate to oxalacetic acid,which increases the number of carbon skeletons of amino acids.