Oligochitosan induces cell death and hydrogen peroxide accumulation in tobacco suspension cells |
| |
Authors: | Wenxia Wang Shuguang Li Yuguang Du Bincheng Lin |
| |
Affiliation: | a Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Natural Products and Glycoconjugate Research Group 1805, Dalian 116023, PR China b Graduate School of the Chinese Academy of Sciences, Beijing 100049, PR China |
| |
Abstract: | Oligochitosan has been shown to induce several plant defense responses. In the present work, the effect of oligochitosan on tobacco cell survival was investigated. The results showed that oligochitosan caused tobacco cell death in a dose-dependent manner. About 40.6 % tobacco cells died when cultured for 72 h after 500 μg ml−1 oligochitosan treatment. Certain aspects of this cell death process appeared to be similar to apoptosis in animal cells. These included shrinkage of cytoplasm and condensation of chromatin. Oligochitosan also induced H2O2 accumulation in tobacco cell suspension culture. The role of H2O2 in the signal transduction that leads to cell death was investigated. Co-treatment of tobacco cells with oligochitosan and catalase inhibited H2O2 accumulation but did not inhibit the induction of cell death. The results suggested that apoptosis-like cell death of tobacco cells induced by oligochitosan is independent of H2O2 signal pathway. |
| |
Keywords: | Oligochitosan Apoptosis Hydrogen peroxide Plant defense responses |
本文献已被 ScienceDirect 等数据库收录! |