| RBSDV-S10基因和SCMV-CP基因双价植物表达载体的构建 |
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| 引用本文： | 王迅,江蓓蓓,李晓颖,陈妮,公娇芬,竺晓平. RBSDV-S10基因和SCMV-CP基因双价植物表达载体的构建[J]. 山东农业科学, 2010, 0(8): 1-4 |
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| 作者姓名： | 王迅 江蓓蓓 李晓颖 陈妮 公娇芬 竺晓平 |
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| 作者单位： | 山东农业大学植物保护学院,山东,泰安,271018 |
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| 基金项目： | 中国博士后科学基金，山东省博士后创新项目专项基金 |
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| 摘 要： | 克隆了水稻黑条矮缩病毒（RBSDV）的外壳蛋白S10基因的片段和甘蔗花叶病毒（SCMV）的外壳蛋白CP基因的片段,将两个片段在pMD18-T Simple Vector上连接起来。获得的S10-CP片段全长为830bp,将其分别以正向和反向插入植物表达载体pMCG161,构建了含两种不同病毒来源基因的RNAi植物表达载体pMCG161-CP10,为进一步转化玉米,探索利用RNAi技术同时防治玉米矮花叶病和玉米粗缩病奠定了基础。
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| 关 键 词： | 玉米 RNAi S10基因 CP基因 |
Construction of Binary Expression Vector Carrying RBSDV-S10 Gene and SCMV-CP Gene |
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| WANG Xun,JIANG Bei-bei,LI Xiao-ying,CHEN Ni,GONG Jiao-fen,ZHU Xiao-ping. Construction of Binary Expression Vector Carrying RBSDV-S10 Gene and SCMV-CP Gene[J]. Shandong Agricultural Sciences, 2010, 0(8): 1-4 |
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| Authors: | WANG Xun JIANG Bei-bei LI Xiao-ying CHEN Ni GONG Jiao-fen ZHU Xiao-ping |
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| Affiliation: | WANG Xun,JIANG Bei-bei,LI Xiao-ying,CHEN Ni,GONG Jiao-fen,ZHU Xiao-ping(College of Plant Protection,Shandong Agricultural University,Taian 271018,China) |
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| Abstract: | Two fragments from RBSDV-S10 and SCMV-CP were cloned by PCR,and inserted into pMD18-T Simple Vector.The combined sequence of S10-CP from two different viral genes was 830 bp.By using the dsRNA binary vector pMCG161 and sequence S10-CP,a new construct of pMCG161-CP10 was obtained containing inverted repeats.This work is valuable for obtaining the anti-virus transgenic maize resisting to two virus diseases(maize rough dwarf disease and maize dwarf mosaic disease)by the way of RNA interference. |
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| Keywords: | RNAi |
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