不同诱导处理后水稻悬浮细胞的活性氧变化与有关酶系的关系

以水稻（Oryza sativa L.）感病品种浙福802为材料，研究了3种不同因子处理后其悬浮细胞中活性氧及其一些抗病酶系的变化情况。结果表明：(1) 3种因子均能导致H2O2的形成和积累。XOO.75-1处理后的H2O2含量分别在0.5和6 h出现突增现象，而XOO.76-25处理只在0.5～1 h达到一个峰值。推测XOO.75-1和浙辐802间的互作属于非亲和性互

Relationship between the Changes of Active Oxygen Species and Defense Enzymes in Suspension Cultured Cells Treated by Different Inducers

Changes of active oxygen species (AOS) and some defense enzymes in suspension cultured cells of Zhefu 802 (susceptible) induced by three inducers were studied.Three factors all resulted in the accumulation of H 2O 2 in suspension cultured cells.H 2O 2 content in suspension cultured cell treated by XOO.75-1 was increased suddenly at 0.5 hour and at 6 hours after treatment, but that treated with XOO.76-25 only at 0.5 hour to 1 hour after treatment.POD activities in suspension cultured cells treated with XOO.75-1 and XOO.76-25 were both increased by 31.5 % and 49.8 % respectively.But the changes of SOD activities were not marked, even decreased at the beginning.Two new bands (R f=0.48 and R f= 0.72) in SDS-PAGE diagram of soluble protein from suspension cultured cells treated with XOO.75-1 and XOO.76-25 appeared at 72 hours after the induction.In addition, two other bands (R f=0.53, MW=32.2 kD; R f=0.69, MW=41.9 kD) also appeared in the treatment of XOO.75-1.Some isoenzyme bands of POD and SOD in suspension cultured cells treated with three factors were increased or decreased in number, or changed in color.H 2O 2 in suspension cultured cells treated by SA was accumulated, and activities of POD and SOD!increased respectively by 31.5 % and 49.8 %.