不同条件下紫珠种子萌发特征研究

通过设定L9(34)3因素3水平正交试验,探究了萌发温度条件、光照条件和激素(GA3)条件.结果表明:在紫珠发芽率、发芽指数、发芽势和活性指数指标方面,温度是其影响的最主要因子,光照次之,GA3的影响相对最低,综合分析结果显示,紫珠发芽率、发芽指数、发芽势和活性指数指标最优的萌发条件组合为A3 B3 C2,即1000L...

Study on Seed Germination Characteristics of Callicarpa bodinieri Under Different Conditions

The conditions of germination temperature,light and hormone(GA3)were studied by setting L₉(3⁴)3 factor 3 level orthogonal test.The results showed that temperature was the main factor affecting the germination rate,germination index,germination potential and activity index of C.bodinieri followed by light,and GA3 was relatively the lowest.The comprehensive analysis results showed that the best combination of germination conditions for C.bodinieri germination rate,germination index,germination potential and activity index was A₂B₃C₂,as 1000lx light for 12h+dark for 12 h,25℃,GA3 concentration of 100 mg/L.In terms of seedling biomass,seedling hypocotyl length,seedling radicle length and hypocotyl/radicle ratio,light is the most important factor;temperature is the second,and GA3 is the lowest.The results of comprehensive analysis showed that the combination of germination conditions most conducive to the germination index of C.bodinieri seeds was A₂B₃C₂,that is,1000lx light for 24 h,25℃,GA3 Concentration of 100 mg/L.The best germination condition combination for the hypocotyl length index of C.bodinieri seeds was A₂B₃C₁,as 1000lx light for 24 h,25℃,GA3 concentration of 50 mg/L.The best germination condition combination for radicle length index of C.bodinieri seeds was A₂B₃C₂,as 1000lx light for 12h+darkness for 12h,25℃,GA3 concentration of 100 mg/L.The germination condition with the largest hypocotyl/radicle ratio was A2B1C1,as 1000lx light for 12 h+darkness for 12 h,25℃,GA3 concentration of 100mg/L.Conclusion:the optimum germination combination condition of C.bodinieri seeds is A₂B₃C₂,that is,1000lx light for 12 h+darkness for 12 h,25℃,GA3 concentration of 100 mg/L.