黑皮果蔗中碱性蔗糖转化酶基因ShINV6的克隆与表达分析

在高等植物中,蔗糖转化酶广泛参与调节植物的生长发育、生物和非生物胁迫应答等过程,在控制光合产物在不同库组织的分配上起重要作用,是决定作物经济产量的关键酶.本研究以黑皮果蔗'Badila'为实验材料,克隆了一个果蔗蔗茎中碱性转化酶基因ShINV6,并对其序列特征、表达特性及其编码蛋白的酶学特征进行了分析.生物信息学分析发...

Molecular Cloning and Expression Analysis of a Neutral / Alkaline Invertase Gene (ShINV6) from ‘Badila’

In higher plants, invertase is widely involved in regulating plant growth, development, biological and abiotic stress response, and plays an important role in controlling the distribution of photosynthates in different bank tissues. It is a key enzyme that determines crop economic yield. In this study, the neutral/alkaline invertase ShINV6 was cloned from the stem of ‘Badila’. The sequence characteristics, expression characteristics and enzymatic characteristics of its encoding protein were analyzed. Bioinformatics analysis found that the full length of ShINV6 cDNA was 2859 bp, including 1254 bp of ORF that encoding 417 amino acids, and the content of arginine (Arg) was 12.7%. The molecular weight of ShINV6 was 46.29 kD and the PI value was 10.89. Evolutionary relationship analysis showed that ShINV6 protein was highly homologous with the neutral / alkaline invertase of monocotyledon crops such as sugarcane, maize and sorghum. It has typical Glyco_hydro_100 domain, multiple sequence alignments revealed that the C-terminal of ShINV6 was about 130 amino acids less than other invertases. Cell localization and hydrophilic analysis predict that ShINV6 may be hydrophilic protein in cytoplasm. During the growth and development of sugarcane, the change of sucrose content in sugarcane stems was observed. It was found that the sucrose content increased gradually with the extension of planting time, and the most sugar accumulation month was from October to November. QRT-PCR results showed that ShINV6 expression reached its maximum in September, and combined with regulation of biological yield growth in sugarcane growing period, it was speculated that ShINV6 might be involved in the unloading process of sucrose which the product of photosynthesis in the peak growth stage of sugarcane stems (library), and sucrose was transformed into hexaccharide for the rapid growth of sugarcane stems.