| 利用花粉管结合线性DNA的方法将YB2基因导入玉米自交系的研究 |
| |
| 引用本文： | 孙会娟 李 杰 邹华文等. 利用花粉管结合线性DNA的方法将YB2基因导入玉米自交系的研究[J]. 安徽农业科学, 2013, 0(24): 9918-9920,10016 |
| |
| 作者姓名： | 孙会娟 李 杰 邹华文等 |
| |
| 作者单位： | [1]首都师范大学生命科学学院,北京100048;北京市农林科学院北京农业生物技术研究中心北京100097 [2]长江大学农学院,湖北荆州434023 [3]北京市农林科学院北京农业生物技术研究中心,北京100097 |
| |
| 基金项目： | 国家转基因重大专项重点课题（2009ZX08003-009B）;北京市科委项目（Z121100001512012和Zl11100066111005）;北京市农林科学院科研能力创新项目（KJCX201102003） |
| |
| 摘 要： | [目的]将YB2基因导入玉米自交系.[方法]利用花粉管通道法将含有YB2基因的线性表达盒转入优良玉米自交系京24,对T0代植物喷洒浓度为200 mg/L草铵膦药液进行筛选,并对筛选到的植株进行PCR、RT-PCR检测.[结果]试验共得到19株草铵膦抗性植株;经检测,19株均为Bar阳性植物,其中5株含YB2目的基因,初步证明外源目的基因已整合到玉米基因组中.[结论]该研究为获得抗旱性好、产量高、安全的转基因玉米株系奠定了基础.
|
| 关 键 词： | 花粉管通道法 YB2基因 转基因玉米 |
Study on the Transferring Linear of YB2 Gene into Maize Inbred Lines via Pollen Tube Pathway |
| |
| Affiliation: | SUN Hui-juan et al (College of Life Science, Capital Normal University, Beijing 100048) |
| |
| Abstract: | [ Objective/ The aim was to introduce Yb＇2 gene into maize inbred hnes. [ Method J lanear expressing cassette commnmg YB2 gene was transferred into maize inbred line Jing 24 by pollen tube pathway method. To generation plants were screened by spraying 200 mg/L glufosi- nate herbicide,and the screened plants were detected by PCR and RT-PCR. ~ Result] 19 glufosinate-tolerant plants were obtained. All of them were Bar positive plants ,5 of them contained YB2 gene ,which proved that exogenous gene had been integrated into the genome of maize. [ Con- clusion ] The study laid a foundation for obtaining transgenic maize lines with good drought resistance, high yield and good security. |
| |
| Keywords: | Pollen-tube pathway YB2 gene Transgenic maize |
| 本文献已被 CNKI 维普 等数据库收录！ |
