| 利用花粉管结合线性DNA的方法将YB2基因导入玉米自交系的研究 |
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| 引用本文： | 孙会娟,李,杰,邹华文等.利用花粉管结合线性DNA的方法将YB2基因导入玉米自交系的研究[J].安徽农业科学,2013(24):9918-9920,10016. |
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| 作者姓名： | 孙会娟 李 杰 邹华文等 |
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| 作者单位： | [1]首都师范大学生命科学学院,北京100048;北京市农林科学院北京农业生物技术研究中心北京100097 [2]长江大学农学院,湖北荆州434023 [3]北京市农林科学院北京农业生物技术研究中心,北京100097 |
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| 基金项目： | 国家转基因重大专项重点课题（2009ZX08003-009B）;北京市科委项目（Z121100001512012和Zl11100066111005）;北京市农林科学院科研能力创新项目（KJCX201102003） |
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| 摘 要： | 目的]将YB2基因导入玉米自交系.方法]利用花粉管通道法将含有YB2基因的线性表达盒转入优良玉米自交系京24,对T0代植物喷洒浓度为200 mg/L草铵膦药液进行筛选,并对筛选到的植株进行PCR、RT-PCR检测.结果]试验共得到19株草铵膦抗性植株;经检测,19株均为Bar阳性植物,其中5株含YB2目的基因,初步证明外源目的基因已整合到玉米基因组中.结论]该研究为获得抗旱性好、产量高、安全的转基因玉米株系奠定了基础.
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| 关 键 词： | 花粉管通道法 YB2基因 转基因玉米 |
Study on the Transferring Linear of YB2 Gene into Maize Inbred Lines via Pollen Tube Pathway |
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| Institution: | SUN Hui-juan et al (College of Life Science, Capital Normal University, Beijing 100048) |
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| Abstract: | Objective/ The aim was to introduce Yb＇2 gene into maize inbred hnes. Method J lanear expressing cassette commnmg YB2 gene was transferred into maize inbred line Jing 24 by pollen tube pathway method. To generation plants were screened by spraying 200 mg/L glufosi- nate herbicide,and the screened plants were detected by PCR and RT-PCR. ~ Result] 19 glufosinate-tolerant plants were obtained. All of them were Bar positive plants ,5 of them contained YB2 gene ,which proved that exogenous gene had been integrated into the genome of maize. Con- clusion ] The study laid a foundation for obtaining transgenic maize lines with good drought resistance, high yield and good security. |
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| Keywords: | Pollen-tube pathway YB2 gene Transgenic maize |
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