植物生长调节剂对离体培养早熟杏胚休眠的破除

以自然授粉的早熟杏品种焦家杏胚为材料 ,应用植物生长调节剂 6 BA、IAA、GA3 以及活性炭 (AC)对离体培养条件下解除杏胚的休眠进行了研究 ,结果表明 :在 1/ 2MS培养基中附加 0 .5～ 2mg/L的 6 BA可以打破杏胚休眠 ,其中以 2mg/L的 6 BA成苗效果较好 ,成苗率为 83.3% ;在培养基中同时加入 0 .0 2 %AC和 10mg/L的 6 BA效果更好 ,其生长状况 (苗高 )显著好于前者

Break of the Dormancy of Apricot Embryo by Plant Growth Regulators in vitro

The open-pollinated embryos of early-ripening apricot Jiaojiaxing were used for studying the influence of 6-BA,IAA,GA3 and activated charcoal on embryo culture. The results indicated that: when the embryos of Jiaojiaxing were cultured on the 1/2 MS medium, with the addition of 0.5～2 mg/L 6-BA, the dormancy could be abolished, especially when the amount of 6-BA was 2 mg/L, the results were good, the percent of plant formation was 83.3%. When the embryos were cultured on the 1/2 MS medium, with the addition of 0.02% AC and 10 mg/l 6-BA,the results were better. Though the plant formation rates had no significant difference between the two treatments, the growth appearance of the latter was obviously better than the former.