通过粪便确定野生马鹿性别的试验

对已知性别的4份肌肉样品、4份圈养马鹿粪便样品和通过野外马鹿卧迹的排尿位置判断出性别的8份粪便样品进行扩增,其结果与实际性别相吻合。在验证了PCR有效性的基础上,对野外收集的183份马鹿粪便样品中的108份成功鉴定出性别,即:80份为雄性,28份为雌性。同时指出,由于一些粪便DNA样品中存在大量的聚合酶抑制剂,致使无法得到扩增结果。因此,对所有DNA样品均PCR扩增了3次,以提高性别鉴定的准确率和样品的利用率。

Sex Determination of Wild Red Deer by Feces

Multiplex PCR amplification of two microsatellite markers on normal chromosome and the SRY gene were used to identify the sex of wild red deer(Cervus elaphus) by feces samples.Accordant results were gained by comparing four muscle samples and four feces samples from sex-known captive red deer with eight feces samples from the wild red deer whose sexes were detected by their lying and micturating places.The sexes were detected successfully in 108 of 183 feces samples collected in the field.Result showed that there were 80 males and 28 females.The amplification result of some samples could not be obtained due to the inhibition of polymerase in some feces.So,all the samples were amplified three times in order to enhance the precision of sex determination and the use ratio of sample.