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| 两株猪圆环病毒2型的分离及基因组序列分析 | |
| 引用本文： | 王文秀,吕素芳,朱辉,管宇,魏凤,唐娜,沈志强. 两株猪圆环病毒2型的分离及基因组序列分析[J]. 动物医学进展, 2011, 32(9): 5-9 |
| 作者姓名： | 王文秀 吕素芳 朱辉 管宇 魏凤 唐娜 沈志强 |
| 作者单位： | 1. 山东省滨州畜牧兽医研究院,山东滨州,256600 2. 山东绿都生物科技有限公司,山东滨州,256600 3. 山东省滨州畜牧兽医研究院,山东滨州256600;山东绿都生物科技有限公司,山东滨州256600 |
| 基金项目： | 山东省自主创新成果转化重大专项计划(2008ZHZX1A1103) |
| 摘 要： | 为比较猪圆环病毒2型毒株的遗传变异特性,从2009年河南郑州和山东东营的猪场疑似猪断奶后多系统衰弱综合征(PMMS)病料中分离到2株病毒,经PCR检测初步鉴定为猪圆环病毒2型,并对病毒全基因组进行扩增,用DNA Star对序列进行比较分析.结果表明,分离到的河南郑州和山东东营PCV-2毒株全基因组长度均为1 767 b... |
| 关 键 词： | 猪圆环病毒2型 分离 基因组 序列分析 |
Isolation and Genomic Sequence Analysis of Two PCV-2 |
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| WANG Wen-xiu,L Su-fang,ZHU Hui,GUAN Yu,WEI Feng,TANG Na,SHEN Zhi-qiang. Isolation and Genomic Sequence Analysis of Two PCV-2[J]. Progress In Veterinary Medicine, 2011, 32(9): 5-9 | |
| Authors: | WANG Wen-xiu L Su-fang ZHU Hui GUAN Yu WEI Feng TANG Na SHEN Zhi-qiang |
| Affiliation: | WANG Wen-xiu,L(ü) Su-fang,ZHU Hui,GUAN Yu,WEI Feng,TANG Na,SHEN Zhi-qiang |
| Abstract: | Two porcine circovirus type 2(PCV-2),strains DY01 and HN01 were isolated from highly pathogenic porcine reproductive and respiratory syndrome virus-infected samples.The PCV-2 isolates were identified by PCR method.The complete genomes of these PCV-2 isolates were sequenced and their molecular variations analyzed using DNAStar software.Comparison analysis of the complete genomic sequences of two PCV-2 isolates indicated that they were closely related to each other(95.9%)and to other PCV-2 strains in GenBank(93.5%-99.6%).There was 68.9% to 69.3% sequence identity among PCV-2 and PCV-1 isolate genes.Phylogenetic analysis revealed that the two isolates belonged to genotype PCV-2b.This two PCV-2 strains isolated from Henan and Shandong provinces were both belong to PCV-2b type,and this two PCV-2 strains have area lvariation on gene evolution. |
| Keywords: | Porcine circovirus type 2 isolation genome sequence analysis |
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