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广西地区副猪嗜血杆菌ERIC-PCR指纹图谱分型研究
引用本文:彭昊,李军,谢宇舟,马春霞,禤雄标,胡帅,杨威,许永干,谢永平,陈泽祥,潘艳. 广西地区副猪嗜血杆菌ERIC-PCR指纹图谱分型研究[J]. 动物医学进展, 2011, 32(11): 49-52
作者姓名:彭昊  李军  谢宇舟  马春霞  禤雄标  胡帅  杨威  许永干  谢永平  陈泽祥  潘艳
作者单位:广西壮族自治区兽医研究所,广西南宁,530001
基金项目:广西壮族自治区科技厅科技攻关项目
摘    要:为探讨肠道细菌基因间重复序列(ERIC)的聚合酶链反应(PCR)技术用于副猪嗜血杆菌基因分型的可行性,对分离自广西地区不同猪场的22株副猪嗜血杆菌进行ERIC-PCR指纹图谱分型研究.结果发现,22株分离株显示出12种指纹图谱,可以区分无法进行血清分型的菌株.表明ERIC-PCR可适用于对副猪嗜血杆菌进行分子流行病学调...

关 键 词:副猪嗜血杆菌  基因间重复序列的聚合酶链反应  指纹图谱  分型

Genotyping of Haemophilus Parasuis Isolates from Guangxi by ERIC-PCR Fingerprinting
PENG Hao,LI Jun,XIE Yu-zhou,MA Chun-xia,XUAN Xiong-biao,HU Shuai,YANG Wei,XU Li-gan,XIE Yong-ping,CHEN Ze-xiang,PAN Yan. Genotyping of Haemophilus Parasuis Isolates from Guangxi by ERIC-PCR Fingerprinting[J]. Progress In Veterinary Medicine, 2011, 32(11): 49-52
Authors:PENG Hao  LI Jun  XIE Yu-zhou  MA Chun-xia  XUAN Xiong-biao  HU Shuai  YANG Wei  XU Li-gan  XIE Yong-ping  CHEN Ze-xiang  PAN Yan
Affiliation:(Guangxi Veterinary Research Institute,Nanning,Guangxi,530001,China)
Abstract:In order to study the feasibility of ERIC-PCR assay on genotyping of Haemophilus par asuis,22 Haemophilus parasuis isolates from different pig herds in Guangxi were characterised by ERIC-PCR fingerprinting.It was found that the 22 field isolates of H.parasuis presented 12 special fingerprints and all isolates were typable by the method even serologically non-typable isolates.The results suggested that ERIC-PCR fingerprinting is useful for molecular epidemiological survey and the classification of H.parasuis.
Keywords:Haemophilus parasuis  ERIC-PCR  fingerprint  genotype
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