不同氮源对一株溶藻弧菌好氧反硝化效率的影响

为研究溶藻弧菌Vibrio alginolyticus HA2同步硝化反硝化过程中氮的代谢产物,分别用以铵态氮(NH_4~+-N)、硝态氮(NO_3~--N)、亚硝态氮(NO_2~--N)为氮源的培养基培养溶藻弧菌HA2 120 h,测定不同时间段菌液浓度,以及NH_4~+-N、NO_3~--N、NO_2~--N、pH和发酵罐中气体(N_2、NO、N_2O)的含量,并且拟合菌株生长曲线。结果表明:溶藻弧菌对NH_4~+-N、NO_3~--N、NO_2~--N降解率最高分别为99.97%、99.95%、36.87%;生长极限k值分别为4.769、5.477、5.567;培养基中的NH_4~+-N直接被氧化为NO_3~--N;试验中均未检测出NO、N_2O气体,各培养基中N_2量均有上升趋势;各培养基中pH均有增加趋势。研究表明,溶藻弧菌HA2具有开发为高效、环保、安全的硝化反硝化细菌的研究价值。

Effects of different nitrogen culture media on growth and nitrogen metabolites of Vibrio alginolyticus

The concentrations of bacterium Vibrio alginolyticus HA2,and levels of NH+4-N,NO-3-N,and NO-2-N, and pH in the culture medium and levels of N2,NO,and N2O in a fermentation tank were determined in the bacte-rium media containing ammonium nitrogen(NH+4-N),nitrate (NO-3-N),and nitrite (NO-2-N) for 120 h at vari-ous culture periods and the growth curve fitting of Vibrio alginolyticus HA2 was fitted in order to evaluate the effects of different nitrogen culture media on nitrogen metabolites in simultaneous nitrification and denitrification of Vibrio alginolyticus HA2. Results showed that the maximal degradation rate was found to be 99.97% in NH+4-N,99.95%in NO-3-N and 36.87% in NO-2-N, with growth limit K of 4.769 in NH+4-N, 5.477 in NO-3-N, and 5.567 in NO-2-N. The NH+4-N was oxidized to NO-3-N directly in the culture media,and no increase in levels of NO,N2O, and N2were observed. There was elevated pH in the culture media. The findings indicate that Vibrio alginolyticus HA2 will have potential for nitrification and denitrification in efficient protection of environmental safety.