低温胁迫对胡芦巴幼苗抗氧化酶活性和膜脂过氧化的影响 |
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引用本文: | 李慧,张昕欣,彭立新,阎国荣. 低温胁迫对胡芦巴幼苗抗氧化酶活性和膜脂过氧化的影响[J]. 安徽农业科学, 2010, 38(26): 14258-14259,14271. DOI: 10.3969/j.issn.0517-6611.2010.26.025 |
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作者姓名: | 李慧 张昕欣 彭立新 阎国荣 |
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作者单位: | 天津农学院园艺系,天津,300384;沈阳农业大学,土地与环境学院,辽宁沈阳,100866 |
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基金项目: | 天津市农业科技成果转化与推广项目,天津农学院科学研究发展基金计划 |
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摘 要: | [目的]从抗氧化能力的角度揭示胡芦巴幼苗抗寒机理。[方法]以胡芦巴幼苗为材料,研究0℃低温胁迫下胡芦巴幼苗叶和根中抗氧化酶SOD、CAT、POD活性及丙二醛(MDA)含量的变化。[结果]随着低温处理时间的延长,胡芦巴幼苗SOD、POD活性呈现上升-下降-上升趋势,活性在6h处达到小高峰后下降,12h之后又开始上升,并于48h时达最大值;CAT活性呈现先升后降趋势,在12h时达到峰值。叶中抗氧化酶活性变化幅度及峰值均大于根。MDA含量呈现先升后降趋势,在处理24h时达到峰值,且叶中MDA含量小于根。[结论]三种抗氧化酶协同作用抵抗低温胁迫的伤害;叶对低温胁迫的响应反应比根强。
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关 键 词: | 胡芦巴 低温胁迫 抗氧化酶 丙二醛 |
Effects of Cold Stress on Activities of Antioxidant Enzymes and Membrane Lipid Peroxidation of Trigonella foenum-graecum L.Seedlings |
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Affiliation: | LI Hui et al(Department of Horticultural,Tianjin Agricultural University,Tianjin 300384) |
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Abstract: | [Objective] The purpose was to seek for the effect of cold stress on activities of antioxidant enzymes and membrane lipid peroxidation of Trigonella foenum-graecum L.seedlings.[Method] The activities of SOD,CAT,POD and the content of MDA of Trigonella foenum-graecum L.seedlings were studied under 0 ℃ cold stress.[Result] The results showed that,as the treatment time increasing,the activities of SOD and POD showed the increasing-decreasing-increasing tendency:got the small peak at 6h,then decreased till 12 h,and then increased and got the largest activities at 48 h.The activities of CAT showed the increasing-decreasing tendency,and got the peak at 12 h.Compared with roots,the activities of SOD,POD,CAT in the leaves were higher,and the changing trends were more sharply.The contents of MDA showed the increasing-decreasing tendency,and got the peak at 24 h.The contents of MDA in the leaves were lower than those in the roots.[Conclusion] Three antioxidant enzymes work together to resist the cold injury;the response reactions to cold stress in the leaves were more sharply than those in the roots. |
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Keywords: | Trigonella foenum-graecum L. Cold stress Antioxidant enzymes MDA |
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