| 乙酰丁香酮浓度和共培养pH对大豆再生频率的影响 |
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| 引用本文： | 武小霞,李静,王志坤,刘珊珊,李海燕,马永,李文滨.乙酰丁香酮浓度和共培养pH对大豆再生频率的影响[J].东北农业大学学报,2010,41(5). |
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| 作者姓名： | 武小霞 李静 王志坤 刘珊珊 李海燕 马永 李文滨 |
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| 作者单位： | 东北农业大学农学院,大豆生物学省部共建教育部重点实验室,哈尔滨,150030;七台河市新兴区长兴乡人民政府农业综合服务中心,黑龙江,七台河,154532 |
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| 基金项目： | 转基因生物新品种培育重大专项，哈尔滨市科技创新人才研究专项资金项目，东北农业大学博士启动基金 |
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| 摘 要： | 农杆菌介导方法是较成熟的遗传转化方法,由于大豆的特殊性使其在转化过程中受很多因素的影响,为了提高大豆的转化频率,对影响农杆菌介导方法转化大豆转化率最大的乙酰丁香酮(AS)和pH两个重要因子进行了研究。结果表明,在农杆菌侵染和共培养阶段的乙酰丁香酮浓度和pH均对大豆转化频率有明显影响,pH5.4～5.6为宜;乙酰丁香酮能显著提高大豆的转化频率,但浓度不宜过高,浓度过高反而降低转化效率,最佳浓度为100μmol·L-1。
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| 关 键 词： | 大豆 遗传转化 pH 乙酰丁香酮(AS) |
Effect of AS concentration and pH on soybean genetic transformation |
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| WU Xiaoxia,LI Jing,WANG Zhikun,LIU Shanshan,LI Haiyan,MA Yong,LI Wenbin.Effect of AS concentration and pH on soybean genetic transformation[J].Journal of Northeast Agricultural University,2010,41(5). |
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| Authors: | WU Xiaoxia LI Jing WANG Zhikun LIU Shanshan LI Haiyan MA Yong LI Wenbin |
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| Abstract: | Agrobacterium-mediated transformation of soybean has been accepted by most of researchers.Meanwhile,many factors affected the transformation efficiency.The two important factors,acetosyringone(AS) concentration and pH,were assessed in this research.The results showed that pH and acetosyringone concentration in the Agrobacterium infection and co-culture affected the transformation efficiency significantly.The appropriate pH was from 5.4 to 5.6 and the best acetosyringone concentration was 100 μmol·L-1. |
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| Keywords: | pH |
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