Effect of dietary oxidized fish oil and vitamin C supplementation on growth performance and reduction of oxidative stress in Red Sea Bream Pagrus major

A 50‐day feeding trial was conducted to determine the effects of dietary oxidized fish oil (OFO) and vitamin C (VC) on growth and oxidative stress in juvenile red sea bream. Test diets were formulated with 2 degrees of peroxide value (23 and 29 meq kg^?1) combined with 3 levels of VC (0, 400 and 800 ppm). No significant difference was found on growth performance between fish fed OFO with 400 or 800 mg VC and the control group that fed a diet with fresh fish oil after 50 days. However, fish fed OFO without VC supplement indicated significantly poor growth than the control group. Liver and muscle thiobarbituric acid reactive substances were reduced by increased VC intake of fish. Fish fed diets containing low OFO with 400 and 800 mg VC, high OFO with 800 mg VC, and fresh fish oil are allocated in the zone of high resistance against oxidative stress together with low oxidative stress condition. On the other hand, no VC supplemented group was under the highest oxidative stress condition. In conclusion, dietary oxidized lipid increased the oxidative stress condition of fish, but more than 400 mg VC kg^?1 of dietary supplement improved growth and health of juvenile red sea bream.