| 一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定 |
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| 引用本文： | 万春和,施少华,程龙飞,陈红梅,傅光华,张大丙,林芳,林建生,黄瑜. 一种引起种(蛋)鸭产蛋骤降新病毒的分离与初步鉴定[J]. 福建农业学报, 2010, 25(6) |
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| 作者姓名： | 万春和 施少华 程龙飞 陈红梅 傅光华 张大丙 林芳 林建生 黄瑜 |
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| 作者单位： | 1. 福建省农业科学院畜牧兽医研究所,福建福州,350013
2. 中国农业大学动物医学院,北京,100193 |
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| 摘 要： | 自表现产蛋骤降的病死种鸭中无菌采集卵巢以番鸭胚分离到病毒,经对分离的病毒(WR株)进行形态学观察、理化特性测定,发现该病毒有囊膜,直径为30～50nm;对氯仿处理敏感;在pH值为7.2条件下,不凝集鸡、鸭和鹅红细胞。人工感染开产麻鸭能复制出与自然感染病例相同的临床症状和病理变化,并能回收到病毒。应用RT PCR技术对分离毒进行检测,可扩增到约1kb特异性条带。经测序分析表明,所获得的核苷酸序列第9-777位与坦布苏病毒(TMUV)的同源性最高,为88.7%;遗传进化分析表明WR株病毒与坦布苏病毒、圣路易斯脑炎病毒(SLEV)、以色列火鸡脑膜炎病毒(ITV)、恩塔亚病毒(NTAV)具有相似的祖先,但在系统发生树上处于单独的进化分支。由此推测,WR株病毒不属于现有黄病毒科黄病毒属任何一种,为黄病毒属中的新成员,为国内外首次报道。
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| 关 键 词： | 种(蛋)鸭 产蛋骤降 黄病毒 新种 分离与鉴定 |
A newly identified Flavivirus Virus causing abrupt egg-laying reduction in ducks |
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| WAN Chun-he,SHI Shao-hua,CHENG Long-fei,CHEN Hong-mei,FU Guang-hua,ZHANG Da-bing,LIN Fang,LIN Jian-sheng,HUANG Yu. A newly identified Flavivirus Virus causing abrupt egg-laying reduction in ducks[J]. Fujian Journal of Agricultural Sciences, 2010, 25(6) |
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| Authors: | WAN Chun-he SHI Shao-hua CHENG Long-fei CHEN Hong-mei FU Guang-hua ZHANG Da-bing LIN Fang LIN Jian-sheng HUANG Yu |
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| Affiliation: | WAN Chun-he1,SHI Shao-hua1,CHENG Long-fei1,CHEN Hong-mei1,FU Guang-hua1,ZHANG Da-bing2,LIN Fang1,LIN Jian-sheng1,HUANG Yu1(1.Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China,2.College of Veterinary Medicine,China Agriculture University,Beijing 100193,China) |
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| Abstract: | A virus WR strain was isolated from the ducks,which experienced abrupt reduction in egg-laying or died of ovarianhemorrhage.The Muscovy ducks inoculated with the WR strain showed pathological effects similar to that of the field cases.The virus could be recovered from the tissues of the sampled ducks in the wild.The viron of the isolated virus had an enveloping membrane and was 30-50 nm in diameter,as observed under electron microscope.The virus WR strain was sensitive to chloroform,and had no haemagglutina... |
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| Keywords: | Laying ducks egg-laying reduction Flavivirus new species isolation and identification |
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