13/17罗伯逊易位纯合子、杂合子猪及3个引进品种核仁组成区(Ag-NORs)的多态性

对 1 3/ 1 7易位纯合子猪、杂合子猪及杜洛克、长白、约克夏猪的核仁组成区 ( Ag-NORs)的多态性作了研究 ,结果表明 ,杜洛克、约克夏猪 Ag-NORs定位于 8号和 1 0号染色体的次缢痕区 ,而 1 3/ 1 7易位纯合子猪、1 3/ 1 7易位纯合子猪×约克夏猪 F1代 Ag-NORs的遗传方式符合孟德尔定律 ;Ag-NORs在细胞间、个体间及品种间数目均有差异 ,而且品种差异显著大于品种内个体间差异。 1 3/ 1 7易位纯合子猪、杂合子猪 ,以及长白、杜洛克、约克夏猪 Ag-NORs均数分别为 1 .99、1 .98、2 .0 0、2 .0 5、2 .0 6,说明 Ag-NORs在品种、个体及细胞间具有多态性。这种多态性与猪的起源和杂交育种时所用亲本品种的血缘成分有关 ,因此可作为遗传标记 ,用以分析家畜的亲缘关系

Polymorphism of Chromosome Ag NORs in Homozygous and Heterozygous Pigs of 13/17 Robertsonian Translocation, Landrace, Yorkshire and Duroc

Polymorphism of Chromosome Ag NORs in the homozygous and heterozygous pigs of 13/17 Robertsonian translocation, Landrace, Yorkshire and Duroc were analyzed.Ag NORs were located on the secondary constrictions of chromosome No.8 and No.10 in Duroc and Yorkshire,but in Landrace,Ag NORs was only located on the secondary constriction in the short arm of chromosome No.10.According to the difference in number and the diversity of the NOR among the homozygous of 13/17 Robertsonian translocation,Yorkshire and their F1,inheritance of NOR conformed to the Mendelian law.The number of Ag NORs was different with strains, individuals and cells, and the difference among strains was much bigger than that among individuals in a strain.The mean mumbers of Ag NORs in homozygous and heterozygous pigs of 13/17 Robertsonian translocation,Duroc,Yorkshire,Landrace were 1.99,1.98,2.05,2.06 and 2.00 respectively.It is suggested that Ag NORs have polymorphism among strains,which can be used as a genetic index to analyze relationships among domestic animals with high reliability.