| 利用嫁接提高花生离体再生或转基因苗成活率的研究 |
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| 引用本文： | 李长生,夏 晗,卢金东,李爱芹,赵传志,毕玉平,王兴军.利用嫁接提高花生离体再生或转基因苗成活率的研究[J].中国农学通报,2009,25(20):63-67. |
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| 作者姓名： | 李长生 夏 晗 卢金东 李爱芹 赵传志 毕玉平 王兴军 |
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| 作者单位： | 1. 山东省农业科学院高新技术研究中心,农业部黄淮海作物遗传改良与生物技术重点开放实验室山东省作物与畜禽品种改良生物技术重点实验室,济南,250100
2. 山东省农业科学院蔬菜研究所,济南,250100 |
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| 基金项目： | 国家高技术研究发展计划项目"花生脂肪酸代谢相关基因的分离与功能研究"，山东省农业科学院高技术自主创新基金"花生籽粒全长Cdna库的大规模测序及脂肪酸合成相关键酶基因的分离和功能分析"(2006YCX030):"花生品质相关功能基因挖掘及高效转基因技术体系研究" |
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| 摘 要： | 花生（Arachis hypogaea L.）是世界上重要的油料作物。近年来，花生分子生物学发展迅速，但低效的再生和遗传转化系统严重制约着利用基因工程改良花生品种的研究。利用嫁接技术将组培苗或转基因苗嫁接到实生苗砧木上，不同嫁接方法成活率达到70-100%，远远高于转基因幼苗直接诱导生根移栽的成活率。
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| 关 键 词： | Cd2+ Cd2+ Pb2+ 木麻黄 萌发 MDA SOD |
| 收稿时间： | 2009-04-23 |
| 修稿时间： | 2009-07-10 |
Graft Significantly Improves Survival Rate of Transgenic Peanut Plants |
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| Li Changsheng,Xia Han,Lu Jindong,Li Aiqin,Zhao Chuanzhi,Bi Yuping,Wang Xingjun.Graft Significantly Improves Survival Rate of Transgenic Peanut Plants[J].Chinese Agricultural Science Bulletin,2009,25(20):63-67. |
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| Authors: | Li Changsheng Xia Han Lu Jindong Li Aiqin Zhao Chuanzhi Bi Yuping Wang Xingjun |
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| Institution: | Li Changsheng, Xia Han, Lu Jindong, Li Aiqin, Zhao Chuanzhi, Bi Yuping, Wang Xingjun (1 High-Tech Research Center, Shandong Academy of Agricultural Science, Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Key Laboratory for Genetic Improvement of Crop, Animal and Poultry of Shandong Province, Ji＇nan 250100; Wegetables Research Institute, Shandong Academy of Agricultural Science, Ji＇nan 250100) |
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| Abstract: | Peanut (Arachis hypogaea L.) is one of the most important oil crops in the world, belonging to the Leguminosae family and the Papilionacea subfamily, native to South America. Inefficiency of peanut regeneration system retarded its improvement through gene engineering. In this study we investigated the use of grafting to improve the survival rate of tissue cultured and transgenic plants. Plants of 14 days after germination were used as rootstock, and different grafting methods and plant management after grafting were investigated. The survival rate of different grafting methods ranged from 70-100% which is much higher than the survival rate obtained by directly transplanting transgenic plants after root induction. |
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| Keywords: | Arachis hypogaeazz Graftingzz Tissue culturezz Gene transformationzz Survival ratezz |
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