| 海藻酸钠裂解酶产生菌的筛选及酶活性研究 |
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| 引用本文： | 武玉永,谭秀华,姚庆收.海藻酸钠裂解酶产生菌的筛选及酶活性研究[J].安徽农业科学,2010,38(3):1126-1128,1148. |
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| 作者姓名： | 武玉永 谭秀华 姚庆收 |
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| 作者单位： | 滨州医学院遗传学教研室,山东烟台,264003;滨州医学院遗传学教研室,山东烟台,264003;滨州医学院遗传学教研室,山东烟台,264003 |
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| 基金项目： | 滨州医学院科技计划项目(BY2007KJ14) |
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| 摘 要： | 目的]筛选海藻酸钠高效降解菌并研究其产酶活性。方法]以海藻酸钠为唯一碳源，通过驯化培养、初筛、复筛得到一株优势菌，并对该菌的最适生长条件、最适产酶条件及酶的性质作了初步研究。结果]优选出1株海藻酸钠高效降解菌8号菌株，为革兰氏阴性菌，形态为杆状。该菌在30℃培养72h产酶量最高。海藻酸钠裂解酶作用的最适底物质量分数为1．00％～2．00％，最适pH值为7．o，最适反应温度为4JD℃，温度继续升高，酶活力急剧下降。结论]为海藻酸钠的有效降解提供了科学依据。
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| 关 键 词： | 褐藻胶降解酶 筛选 最适温度 最适pH值 酶学性质 |
Screening of A Strain Producing Alginate Sodium Lyase and Preliminary Research of Enzyme Activity |
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| WU Yu-yong et al.Screening of A Strain Producing Alginate Sodium Lyase and Preliminary Research of Enzyme Activity[J].Journal of Anhui Agricultural Sciences,2010,38(3):1126-1128,1148. |
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| Authors: | WU Yu-yong |
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| Institution: | Gentetics Staff Room;Binzhou Medical College;Yantai;Shandong 264003 |
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| Abstract: | Objective] The aim was to screen high efficient alginate lyase and study its enzyme activities.Method]A dominant bacteria had got through domestication,screening,and rescreened using alginate sodium as a sole source of carbon.The dominant bacterias of optimal growth conditions,the conditions for enzyme production and the characterization of enzyme activity were studied respectively.Result] One high efficient alginate lyase No.8 strain was optimized,which is gram-negative bacteria and rod-shaped.The bacte... |
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| Keywords: | Alginate lyase Screening Optimum temperature Optimum pH value Enzymatic properties |
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