肝素浓度和作用时间对绵羊精子体外获能和体外受精的影响

摘 要：通过研究添加不同的肝素浓度(0、5、10、20、50μg/ml)及不同作用时间（0、30、45、60、90、120min）对绵羊精子体外获能、存活时间和相应体外受精的影响，为改善绵羊精子体外获能效果及研究获能的机理提供一定的参考。在获能液中添加10μg/ml肝素作用45min时，其获能率最高，顶体反应率最低（40.92％和16.59％）（P<0.05），存活时间18h，能够提高受精卵的囊胚发育；单独使用咖啡因或与10μg/ml肝素联合使用，其获能效果均不如单独使用10μg/ml肝素。说明绵羊精子获能时肝素的适宜浓度是10μg/ml，作用时间是45min，咖啡因无协同肝素促进获能的作用。

Effects of Heparin Dosage and Sperm Capacitation Time on Ovine Sperm Capacitation and Fertilization in vitro

In order to find out some modified methods of ovine sperm capacitation in vitro,the effects of their sperm capacitation,motility,enduring durations and fertilization after respectively adding with 0,5,10,20,50 μg/mL heparin at different time （0,30,45,60,90,120 min）,were compared and analyzed.The results showed that ovine sperm capacitation rate was highest （40.92%）,acrosome reaction was lowest （16.59%）,enduring durations were longest （18 h）,and the blastula rate was highest,when adding with 10 μg/mL heparin in the capacitation medium for 45 min.The effects of capacitation when adding with caffeine or caffeine and 10 μg/mL heparin,were not as good as the effects adding with 10 μg/mL heparin alone.It can be recommended that it is applicable for improving ovine capacitation in vitro system with 10 μg/mL heparin for 45 min,caffeine don ＇ t cooperate with heparin to improve the rate of capacitation.