| 毛白杨PtDREB2A基因的克隆、表达及单核苷酸多态性分析 |
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| 引用本文： | 郭琦,王保垒,王博文,李百炼,张德强. 毛白杨PtDREB2A基因的克隆、表达及单核苷酸多态性分析[J]. 林业科学, 2011, 47(4) |
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| 作者姓名： | 郭琦 王保垒 王博文 李百炼 张德强 |
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| 作者单位： | 北京林业大学林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室,北京,100083 |
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| 基金项目： | 国家林业公益性行业科研专项经费项目(201004009); 教育部新世纪优秀人才支持计划专项基金(NCET-07-0084) |
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| 摘 要： | 利用基因特异的PCR引物由毛白杨受干旱胁迫后构建的cDNA文库中扩增获得一PtDREB2A cDNA克隆,并进行测序和序列分析,结果表明:PtDREB2A cDNA片段总长为946 bp,基因内部含有完整的开放阅读框架,大小为864 bp,可编码长度为287个氨基酸残基的蛋白质,所推导的蛋白质氨基酸序列在AP2/ERF结构功能域与拟南芥AtDREB2A和水稻OsDREB2A蛋白的同源性分别为80.3%和83.3%。组织特异性Realtime-PCR结果显示,PtDREB2A在杨树根、茎、叶片和顶端分生组织中均有表达,但其表达模式不同:PtDREB2A在叶片表达丰度最高,在树干皮层及根部组织表达丰度中等,而在顶端分生组织及主干韧皮部、形成层与木质部表达丰度最低。非生物胁迫及激素诱导差异表达显示,PtDREB2A不仅受高温、低温、干旱与盐诱导表达,还受到激素IAA,NAA及GA3的上调表达,但与ABA的诱导无关。组合利用MEGA4.0和DnaSP4.50.4软件对毛白杨45株基因型个体的PtDREB2A序列进行比对和分析,共检测到49个单核苷酸多态性(SNP)位点,SNP频率为1/18 bp,多样性指数π为0.0...
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| 关 键 词： | 毛白杨 DREB2A基因 非生物胁迫 单核苷酸多态性 |
Isolation, Expression and Single Nucleotide Polymorphisms Analysis of PtDREB2A in Populus tomentosa |
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| Guo Qi,Wang Baolei,Wang Bowen,Li Bailian,Zhang Deqiang. Isolation, Expression and Single Nucleotide Polymorphisms Analysis of PtDREB2A in Populus tomentosa[J]. Scientia Silvae Sinicae, 2011, 47(4) |
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| Authors: | Guo Qi Wang Baolei Wang Bowen Li Bailian Zhang Deqiang |
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| Affiliation: | Guo Qi Wang Baolei Wang Bowen Li Bailian Zhang Deqiang (National Engineering Laboratory for Tree Breeding Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083) |
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| Abstract: | A cDNA clone encoding DREB2A was isolated from a cDNA library prepared under drought stress by the gene-specific PCR amplification from Populus tomentosa.The cDNA was 946 bp in length with an open reading frame (ORF,864 bp in length) which is capable of encoding a protein of 287 amino acids.The deduced protein sequence of the PtDREB2A shares 80.3%,and 83.3% identity with functional domain of AP2/ERF of Arabidopsis thaliana AtDREB2A,and Oryza sativa OsDREB2A,respectively.Tissue differential expression detect... |
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| Keywords: | Populus tomentosa DREB2A gene abiotic stress single nucleotide polymorphisms |
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