应用RAPD技术研究4种鲍的亲缘关系

应用RAPD技术研究 4种鲍的亲缘关系结果表明 ,4种鲍群体中 2 0个有效引物共扩增出 5 38条DNA带 ,平均每个引物扩增的条带数为 2 6 .9;扩增的多态性条带数 136 ,平均每个引物扩增的多态性条带数为 6 .8;多态位点百分数为 2 5 .3%。盘鲍群体和皱纹盘鲍群体之间遗传距离与遗传一致度分别为 0 .2 8和 0 .72 ,杂色鲍群体和九孔鲍群体之间遗传距离与遗传一致度分别为 0 .32和 0 .6 8。聚类分析把盘鲍群体和皱纹盘鲍群体聚为 1组 ,二者亲缘关系较近 ;杂色鲍群体和九孔鲍群体聚为 1组 ,二者亲缘关系也较近

Study on genetic relationship among 4 kinds of abalone using RAPD technique

RAPD technique is used to study the genetic relationship among 4 kinds of abalone. The results show that total 538 DNA bands are scoped by 20 effective primers in 4 kinds of abalone, of which 136 DNA bands are reproducibly polymorphic, and the percentage of polymorphic products is 25.3%; the genetic distance and genetic identity between Haliotis discus discusand H.discus hannai are 0.28, 0.72 respectively, those between H.diversicolor diversicolorand H.diversicolor supertextaare 0.32, 0.68 respectively. In the results of cluster analysis, H.discus discusand H.discus hannaiare pooled into one group, they both are genetically closer; H.diversicolor diversicolorand H.diversicolor supertextaare pooled into another group, they both also are genetically closer.