| Abstract: | Chromatographic distinction of humic and non humic substances in chernozem-humus Fulvic acids, brown humic acids and grey humic acids from a chernozem from the Austrian Marchfeld were analyzed chromatographically on controlled pore glass with pore diameters of 177 and 259 Å. Concentration of coloured material and concentration of carbon were recorded in the chromatogramms. Specific extinctions at 400 nm were calculated for unit of C and maximal values were taken as constants specific for “pure” humic substances. These were 445,6 ± 10,5,388,7 ± 19,3 and 128,9 ± 0,8 for “pure” grey humic acids, brown humic acids and fulvic acids, respectively. With the aid of these constants, “pure” humic substances could be distinguished from accompanying non humic substances. “Pure” humic substances amounted to 63%, non humic substances to 37% of extractable organic C. 53%, 22% and 25% of the carbon of “pure” humic substances were from grey humic acids, brown humic acids and fulvic acids, respectively. |
