| 番茄叶片小RNA提取方法的优化 |
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| 引用本文： | 陈华,昌伟,杨瑞,王绍辉.番茄叶片小RNA提取方法的优化[J].北京农学院学报,2012,27(3):4-6. |
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| 作者姓名： | 陈华 昌伟 杨瑞 王绍辉 |
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| 作者单位： | 北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206;北京农学院植物科学技术学院,北京,102206 |
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| 基金项目： | 国家自然科学基金项目，北京市自然科学基金项目 |
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| 摘 要： | 以番茄叶片为材料提取小RNA,比较分析UNlQ-10柱式Trizol总RNA抽提试剂盒+LiCl法,常规Tr-izol+LiCl法和改进的Trizol法+LiCl法的提取效果。结果表明,改进的Trizol+LiCl法能从番茄叶片中提取高质量的小RNA,总RNA琼脂糖凝胶电泳条带完整清晰,核酸蛋白测定仪显示RNA的浓度为1 026.8ng/μL,A260/A280比值为2.00;A260/A230比值为2.31。15%聚丙烯酰胺-7mol/L尿素凝胶电泳显示小RNA带型区域清晰。用该方法提取的高质量的番茄叶片小RNA符合RT-PCR,Northern blot等分子生物学试验的标准。
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| 关 键 词： | 番茄 叶片 小RNA提取方法 |
Majorization of small RNA extraction methods in tomato leaf |
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| CHEN Hua
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CHANG Wei
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YANG Rui
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WANG Shao-hui.Majorization of small RNA extraction methods in tomato leaf[J].Journal of Beijing Agricultural College,2012,27(3):4-6. |
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| Authors: | CHEN Hua CHANG Wei YANG Rui WANG Shao-hui |
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| Institution: | (College of Plant Science and Technology,Beijing University of Agriculture,Beijing 102206,China) |
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| Abstract: | This experiment used tomato leaf to extract small RNA.UNIQ-10 Column Trizol Extraction Kit+LiCl method,a general Trizol+LiCl method and the improved Trizol+LiCl method were compared on the extraction effect.The results showed that the improved Trizol+LiCl method could extract high-quality small RNA from tomato leaves,Agarose gel electrophoresis of total RNA showed clear and complete strips,nucleic acid protein detector measured the 1 026.8 ng/μL in concentration,2.00 for A260/A280 and 2.31 for A260/A230.15% polyacrylamide-7 M urea gel electrophoresis showed clear small RNA strips area.The high-quality small RNA from tomato leaves by improved Trizol+LiCl method met the criterion for small RNA Northern blot and other molecular biology experiments. |
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| Keywords: | Solanum lycopersicum leaf small RNA extraction methods |
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