| 半胱氨酸合成酶基因CPCSaseA植物表达载体的构建 |
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| 引用本文： | 向白菊,林俊,眭顺照,皮伟,闫明旭,蒋安.半胱氨酸合成酶基因CPCSaseA植物表达载体的构建[J].安徽农学通报,2009,15(13):27-29. |
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| 作者姓名： | 向白菊 林俊 眭顺照 皮伟 闫明旭 蒋安 |
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| 作者单位： | 重庆市畜牧科学院,重庆荣昌,402460;重庆市第一农业学校,重庆,401329;西南大学园艺园林学院,重庆北碚,400716 |
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| 基金项目： | 重庆市科委自然科学基金项目CSTC,2007BB1252 |
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| 摘 要： | 半胱氨酸合成酶是植物硫代谢过程中催化O-乙酰丝氨酸和硫化氢合成半胱氨酸的关键酶。用全长cDNA文库EST随机测序的方法获得了蜡梅的半胱氨酸合成酶胞质异形体基因,构建了全长正义植物表达载体pB I121-CPOASTL。
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| 关 键 词： | 半胱氨酸合成酶 CPCSaseA基因 植物表达载体 |
Construction of the Plant Expression Vector of the Cysteine synthase gene CPCSaseA |
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| Xiang Baiju et al..Construction of the Plant Expression Vector of the Cysteine synthase gene CPCSaseA[J].Auhui Agricultural Science Bulletin,2009,15(13):27-29. |
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| Authors: | Xiang Baiju |
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| Institution: | Chong Qing Academy of Animal Husbandry Sciences;RongChang 402460;China |
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| Abstract: | Cysteine synthase(CSase),a key enzyme of plant sulfur metabolism,catalyzes the formation of Cys from sulfide and O-acetylserine.In this paper,the gene of cytosolic isoform of Cysteine synthase was isolated from Chimonanthus praecox,adopting the method of randomly sequencing the ESTs of full-length cDNA library,Constructed a full-length and sense plant expression vector pBI121-CPOASTL,Laid a solid foundation for further work. |
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| Keywords: | Cysteine synthase CPCSaseA plant expression vector |
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