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A PCR-based assay for differentiating A- and A*-type strains of Xanthomonas citri subsp. citri, the causal agent of Asiatic citrus canker |
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| Authors: | Fatemeh Yousefi Koupaei S Mohsen Taghavi Hiroshi Shiotani Shinji Tsuyumu |
| Institution: | 1. Department of Plant Protection, College of Agriculture, Shiraz University, Shiraz, Iran 2. Citrus Research Division, Kuchinotsu, NARO Institute of Fruit Tree Science, 954 Otsu, Kuchinotsu, Minamishimabara, Nagasaki, 859-2501, Japan 3. Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka, 422-8529, Japan |
| Abstract: | By applying A- and A*-type strains of Xanthomonas citri subsp. citri (Xcc) in a repetitive sequence-based polymerase chain reaction (rep-PCR), two DNA amplicons, one unique to each strain, were evaluated as a probe against the DNA of Xcc strains. Two pairs of primers derived from these amplicons were tested in a PCR analysis. The results confirmed that primers Ms+/Ms? are useful for differentiating A-type from A*-type strains of Xcc. Also, a multiplex PCR with both set of primers can be used to distinguish three groups in Xcc populations: A-type strains and two subgroups of A* strains including Iranian and Thai A* strains. |
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