| 鸡冠花离体快繁及多倍体诱导 |
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| 引用本文： | 王军玲,汪卫星,郭启高,王忠猛,向素琼,李晓林,梁国鲁. 鸡冠花离体快繁及多倍体诱导[J]. 分子植物育种, 2008, 6(1): 187-192 |
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| 作者姓名： | 王军玲 汪卫星 郭启高 王忠猛 向素琼 李晓林 梁国鲁 |
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| 作者单位： | 西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716;西南大学园艺园林学院,重庆,400716 |
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| 摘 要： | 以鸡冠花顶芽为外植体,研究其离体快繁程序并在无菌体繁殖条件下探索了秋水仙素溶液不同浓度和时间处理对鸡冠花的诱变效应.快繁研究结果显示:最适初代培养基为MS 6-BA 1.0 mg/L NAA 0.2 mg/L;以芽和茎段为培养对象诱导产生不定芽的最适培养基分别是MS 6-BA 1.0 mg/L NAA 0.1 mg/L,MS 6-BA1.0 mg/L NAA 0.02 mg/L,最佳生根培养基是1/2 MS IBA 0.4 mg/L.采用浸泡法将鸡冠花营养芽在0.05%、0.1%、0.15%和0.2%不同秋水仙溶液浓度下分别处理12 h、24 h、36 h和48 h,并对诱导处理后获得的再生植株进行鉴定,得出以0.15%秋水仙素溶液浸泡36 h为诱导多倍体的最佳处理组合,诱导率达23.3%.
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| 关 键 词： | 鸡冠花 离体培养 多倍体 秋水仙素诱导 |
in vitro Rapid Propagation and Polyploid Induction of Celosia cristata |
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| Wang Junling,Wang Weixing,Guo Qigao,Wang Zhongmeng,Xiang Suqiong,Li Xiaolin,Liang Guolu. in vitro Rapid Propagation and Polyploid Induction of Celosia cristata[J]. Molecular Plant Breeding, 2008, 6(1): 187-192 |
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| Authors: | Wang Junling Wang Weixing Guo Qigao Wang Zhongmeng Xiang Suqiong Li Xiaolin Liang Guolu |
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| Affiliation: | Wang Junling Wang Weixing Guo Qigao Wang Zhongmeng Xiang Suqiong Li Xiaolin Liang Guolu College of Horticulture , L,scape,Southwest University,Chongqing,400716 |
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| Abstract: | The apical buds of Celosia cristata were used as the explants for the rapid propagation to compare the mutagenic effects treated with colchicines at different concentrations and time duration in tissue culture. The results of the rapid propagation showed that MS+6-BA 1.0 mg/L +NAA 0.2 mg/L would be the optimum medium for generation; MS+6-BA 1.0 mg/L +NAA 0.1 mg/L and MS+6-BA 1.0 mg/L +NAA 0.02 mg/L might be the favorable proliferate medium for adventitious buds obtained from buds and stems; the best rooting... |
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| Keywords: | Celosia cristata in vitro culture Polyploid Induced by colchicine |
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