| 山羊胚胎原始生殖细胞的分离与培养 |
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| 引用本文： | 杨继建,葛秀国,窦忠英. 山羊胚胎原始生殖细胞的分离与培养[J]. 西北农林科技大学学报(自然科学版), 2005, 33(5): 15-18 |
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| 作者姓名： | 杨继建 葛秀国 窦忠英 |
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| 作者单位： | 西北农林科技大学,国家干细胞工程技术研究中心陕西省分中心,陕西,杨凌,712100 |
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| 基金项目： | 国家自然科学基金项目(30070373) |
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| 摘 要： | 选择受精后30～50d的关中奶山羊胎儿为材料,参照小鼠原始生殖细胞(PGCs)分离得到了山羊的PGCs,将其分别培养在STO饲养层上,或与同源山羊成纤维细胞(GEF)及山羊睾丸支持细胞(GSCs)共培养。结果表明,3种方法均能获得类EG细胞。分离得到的类EG细胞在STO饲养层上仅传4代;培养在GEF上的效果最差,传至第3代时丢失;而在GSCs上培养的效果最好,可传6代。
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| 关 键 词： | 山羊 原生殖细胞 胚胎干细胞 |
| 文章编号： | 1671-9387(2005)05-0015-04 |
| 收稿时间： | 2004-12-16 |
| 修稿时间： | 2004-12-16 |
The isolation and cultivation of goat primordial germ cells |
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| YANG Ji-jian,GE Xiu-guo,DOU Zhong-ying. The isolation and cultivation of goat primordial germ cells[J]. Journal of Northwest A&F University（Natural Science Edition）, 2005, 33(5): 15-18 |
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| Authors: | YANG Ji-jian GE Xiu-guo DOU Zhong-ying |
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| Affiliation: | (Shaanxi Branch of National Stem Cells Engineering & Technology,Northwest A & F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | dpc (days postcoitum) Guanzhong goats fetus were selected to isolate the PGCs by isolating the mouse PGCs.Three methods were taken in the experiment:first,PGCs were cultured on the STO feedors;second,PGCs were co cultured with the GEF;third,PGCs were co cultured with the GSCs.The results indicated that EG cells were all able to gain in the three ways.And,PGCs co cultured with GSCs were cultured for 6 passages,PGCs on STO were cultured for 4 passages,but PGCs co cultured with GEF were lost in the third passage. |
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| Keywords: | goat primordial germ cells embryonic stem cell |
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