辣椒茎段离体扦插快繁技术研究

利用五彩椒茎尖培养进行快速繁殖周期短（40~50d），且不存在基因型的差异，但增殖系数较低（每周期扩大1倍）。为了进一步扩大繁殖系数，克服其他外植体培养过程中基因型的影响，利用牛角椒的无菌种苗进行了辣椒茎段培养，筛选出了适宜的诱导茎段萌芽培养基和生根培养基。这两种培养基应用在不同来源的彩色辣椒无菌苗茎段，结果同牛角椒培养结果相似。最终试验结果表明适宜辣椒茎段离体扩繁的培养基为MS无机盐+改良MS有机物+NAA 0.1mg/L+IAA 0.2mg/L+CH 400g/L+AgNO3 3.5mg/L+蔗糖30g/L+琼脂7g/L和MS无机盐+改良MS有机物+IAA 0.5mg/L+GA3 0.25mg/L+CH 400g/L+AgNO3 3.5mg/L+蔗糖30g/L+琼脂7g/L；生根培养基为MS无机盐+B5有机物+NAA 0.1mg/L+IAA 0.2mg/L+AC0.2%+蔗糖30g/L+琼脂7g/L。利用茎段培养不仅大大缩短培养周期，而且可以克服基因型的影响，扩大繁殖系数，提高种苗生产的效率。

Research of in vitro Cutting for Fast-Propagation on Stem of Hot Pepper

Although there were some advantage of short cycle and no difference between genotype on stem of hot pepper for fast-propagation, but the propagation coefficient was lower(1 times per cycle). For expanding propagation coefficient and overcoming influence between genotype in the course of cultivation, Stem of Niujiao pepper asepsis seeding was cultivated in this paper, and the fitting stem bud and rootage substrate were screened out. The effecte of asepsis stem for cultivation on difference source of color pepper was same to Niujiao pepper. The results showed that fitting substrate for in vitro cutting for fast-propagation on stem of hot pepper was MS mineral salt improved MS organic matter NAA 0.1mg/L IAA0.2mg/L CH 400g/L AgNO3 3.5mg/L sucrose 30g/L agar 7g/L, MS mineral salt improved MS organic matter IAA 0.5mg/L GA3 0.25mg/L CH 400g/L AgNO3 3.5mg/L sucrose 3g/L agar 7g/L;rootage substrate was MS mineral salt B5 organic matter NAA 0.1 mg/L IAA 0.2mg/L AC 0.2% sucrose 30g/L agar 7g/L.