| 1株杜仲内生真菌的抗氧化活性分析和菌种鉴定 |
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| 引用本文： | 谢辉,李莉,吴鸣谦,陈双林.1株杜仲内生真菌的抗氧化活性分析和菌种鉴定[J].安徽农业科学,2009,37(1):230-232. |
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| 作者姓名： | 谢辉 李莉 吴鸣谦 陈双林 |
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| 作者单位： | 南京师范大学生命科学学院,江苏南京,210046;南京师范大学生命科学学院,江苏南京,210046;南京师范大学生命科学学院,江苏南京,210046;南京师范大学生命科学学院,江苏南京,210046 |
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| 摘 要： | 目的]研究杜仲内生真菌的抗氧化活性和菌种鉴定。方法]采用组织分离法,筛选杜仲叶片的内生菌种。菌株No.173的总抗氧化活性最高,作为供试菌种。制备它的发酵液提取物,测定了提取物抗氧化能力。对菌株No.173进行了形态和分子鉴定。结果]处理4 d后,处理组的POV值极显著性低于对照。第6天起,处理组的效果明显好于阳性对照组（0.04%Vc和VE）。菌株No.173在PDA平板上的菌落初期为白色,渐变至暗青色,背面褐色,菌落边缘不整齐。序列比对结果显示,菌株No.173 rDNA ITS扩增片段的碱基序列与球毛壳菌序列相似性为100%。结论]杜仲叶片中内生真菌菌株No.173为球毛壳菌,具有明显和稳定的抗氧化活性作用。
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| 关 键 词： | 杜仲 抗氧化活性 球毛壳菌 |
Antioxidant Activity Analysis and Identification of an Endophytic Fungal Strain from Eucommia ulmoides Oliv |
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| XIE Hui et al.Antioxidant Activity Analysis and Identification of an Endophytic Fungal Strain from Eucommia ulmoides Oliv[J].Journal of Anhui Agricultural Sciences,2009,37(1):230-232. |
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| Authors: | XIE Hui |
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| Institution: | XIE Hui et al (College of Life Sciences,Nanjing Normal University,Nanjing,Jiangsu 210046) |
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| Abstract: | Objective] The aim was to study the antioxidant activity and strain identification of an endophytic fungal from Eucommia ulmoides Oliv.Method] The tissue separation method was used to screen the endophytic fungi in the leaves of E.ulmoides.Strain No.173 showed highest antioxidant activity and could be used as tested strain.Its broth extract was prepared to determine its antioxidant activity. Strain No.173 was identified from morphology and molecule.Result] After treatment for 4 d,the POV value of treatme... |
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| Keywords: | Eucommia ulmoides Oliv Antioxidant activity Chaetomium globosum Kunze |
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