Diversity of potential microbial parasites colonizing sclerotia of Macrophomina phaseolina in soil |
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Authors: | A. K. Srivastava D. K. Arora S. Gupta R. R. Pandey M. W. Lee |
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Affiliation: | (1) Laboratory of Applied Mycology, Banaras Hindu University, 221 005 Varanas, India;(2) Department of Agrobiology, Dongguk University, 100 Seoul, Korea |
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Abstract: | The colonization of Macrophomina phaseolina sclerotia by microbial parasites was evaluated in unsterilized field soil at different levels of soil moisture (0,-5, and-10 kPa) and temperature (20, 30, and 40°C). The maximum colonization of sclerotia was recorded in soil held at-5 or-10 kPa at 30–40°C. Trichoderma harzianum isolate 25–92 and Pseudomonas fluorescens isolate 4–92 were recorded as potential sclerotial parasites, and they significantly (P=0.05) reduced the germination of sclerotia by 60–63%. Cells of P. fluorescens and buffer-washed conidia of T. harzianum were completely agglutinated at 28°C with crude agglutinin of M. phaseolina. The ability of different antagonists to parasitize the sclerotia were correlated with the agglutination ability of the antagonists. |
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Keywords: | Macrophomina phaseolina Antagonists Germination Agglutination Pseudomonas fluorescence Trichoderma harzianum |
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